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- W1968890928 abstract "Abstract The human intestinal pathogen Entamoeba histolytica has a number of virulence factors which can cause damage to the host. Transcriptional silencing of the gene coding for one of its major toxic molecules, the amoebapore ( Ehap‐a ), occurred following the transfection of amoebic trophozoites with a plasmid containing the 5′ promoter region of Ehap‐a as well as a truncated segment of a neighboring, upstream SINE1 element that is transcribed from the opposite strand. Silencing was dependent on the presence of the truncated SINE1 sequences. Small amounts of short (∼140 n), ssRNA molecules with homology to SINE1 were detected in the silenced amoeba but no siRNA. The silenced Ehap‐a gene domain had a chromatin modification indicating transcriptional inactivation without any DNA methylation. Removal of the plasmid did not restore transcription of Ehap‐a . Transcription analysis by microarrays revealed that a number of additional genes were silenced and some were also up‐regulated. Transfections of amoeba which already had a silenced Ehap‐a , with a plasmid containing a second gene ligated to the 5′ upstream region of Ehap‐a , enabled the silencing, in‐trans, of other genes of choice. The nonvirulent phenotype of the gene‐silenced amoeba was demonstrated in various assays and the results suggest that they may have a potential use for vaccination. © 2008 IUBMB IUBMB Life, 60(9): 598–604, 2008" @default.
- W1968890928 created "2016-06-24" @default.
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- W1968890928 date "2008-05-20" @default.
- W1968890928 modified "2023-09-30" @default.
- W1968890928 title "Epigenetic transcriptional gene silencing in<i>Entamoeba histolytica</i>" @default.
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- W1968890928 doi "https://doi.org/10.1002/iub.96" @default.
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