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• W1968907319 endingPage "79" @default.
• W1968907319 startingPage "77" @default.
• W1968907319 abstract "Nucleic acids display a very characteristic ultraviolet absorption spectrum with a maximum near 260 nm and a minimum near 230 nm. It was discovered in 1950 that the spectrum of native DNA is much lower than the spectrum calculated from its constituent nucleotides (nt). The observed absorbance strongly increases (hyperchromic effect), not only following hydrolysis, but also following mild treatments, known to respect the phosphoester bonds between nt (and to have little effect on the molecular weight). These results show that DNA has a secondary structure made of secondary valence interactions between the nitrogen bases, and that this secondary structure collapses (DNA denaturation) following mild treatments (low pH, high temperature or, even simply, low salt concentration)." @default.
• W1968907319 created "2016-06-24" @default.
• W1968907319 creator A5030463072 @default.
• W1968907319 date "1993-12-01" @default.
• W1968907319 modified "2023-09-26" @default.
• W1968907319 title "The denaturation of DNA" @default.
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• W1968907319 doi "https://doi.org/10.1016/0378-1119(93)90051-4" @default.
• W1968907319 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8276281" @default.
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