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• W1968914373 endingPage "49" @default.
• W1968914373 startingPage "39" @default.
• W1968914373 abstract "DNA and RNA polymerases evolved to function in specific environments with specific substrates to propagate genetic information in all living organisms. The commercial availability of these polymerases has revolutionized the biotechnology industry, but for many applications native polymerases are limited by their stability or substrate recognition. Thus, there is great interest in the directed evolution of DNA and RNA polymerases to generate enzymes with novel, desired properties, such as thermal stability, resistance to inhibitors, and altered substrate specificity. Several screening and selection approaches have been developed, both in vivo and in vitro, and have been used to evolve polymerases with a variety of important activities. Both the techniques and the evolved polymerases are reviewed here, along with a comparison of the in vivo and in vitro approaches." @default.
• W1968914373 created "2016-06-24" @default.
• W1968914373 creator A5025948470 @default.
• W1968914373 creator A5055062084 @default.
• W1968914373 creator A5073019213 @default.
• W1968914373 date "2005-06-01" @default.
• W1968914373 modified "2023-09-26" @default.
• W1968914373 title "Directed evolution of novel polymerases" @default.
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