Matches in SemOpenAlex for { <https://semopenalex.org/work/W1968950287> ?p ?o ?g. }
- W1968950287 endingPage "586" @default.
- W1968950287 startingPage "578" @default.
- W1968950287 abstract "Activation of the G protein–coupled receptor for parathyroid hormone (PTH)/PTH-related protein (PTHrP) produces homologous desensitization of receptor signaling. We have shown recently that the opossum PTH/PTHrP receptor stably expressed in human embryonic kidney (HEK) 293 cells is phosphorylated upon agonist binding and upon activation of serine/threonine protein kinases (PKA and PKC), an event which for some G protein-coupled receptors has been linked to desensitization. To locate the sites of phosphorylation, mutated forms of the opossum PTH/PTHrP receptor were stably expressed in HEK 293 cells, and ligand-stimulated receptor phosphorylation was evaluated. The five serine and threonine residues of the third cytoplasmic loop of the receptor were not required for receptor phosphorylation. Basal and ligand-induced phosphorylation were, however, completely abolished upon deletion of all but the 16 juxtamembrane residues of the cytoplasmic C-terminal tail of the receptor, even though this truncated receptor resembled the wild-type receptor in its level of expression based on Western blotting and radioligand binding. To identify further the phosphorylation sites, the 129 amino acid C-terminal tail of the rat PTH/PTHrP receptor was expressed in E. coli as a recombinant glutathione S-transferase fusion protein. Elimination of a single PKA consensus site in the tail (serine 491) resulted in ≥90% loss of PKA-mediated phosphorylation, identifying this as the preferential site for PKA, with two other sites (serine 473 and/or 475) being minor sites. Phosphorylation by PKC occurred largely in the proximal portion of the tail, whereas β-adrenergic receptor kinase 1 (βARK1) phosphorylated more distally in the tail. The ability of these kinases to phosphorylate the PTH/PTHrP receptor at distinct sites on the cytoplasmic tail may allow differential regulation of receptor signaling and trafficking." @default.
- W1968950287 created "2016-06-24" @default.
- W1968950287 creator A5029762083 @default.
- W1968950287 creator A5038619650 @default.
- W1968950287 creator A5061689840 @default.
- W1968950287 creator A5067422009 @default.
- W1968950287 creator A5072904368 @default.
- W1968950287 date "2009-12-03" @default.
- W1968950287 modified "2023-09-24" @default.
- W1968950287 title "Phosphorylation of the cytoplasmic tail of the PTH/PTHrP receptor" @default.
- W1968950287 cites W1482398833 @default.
- W1968950287 cites W1504478980 @default.
- W1968950287 cites W1530894594 @default.
- W1968950287 cites W1561488685 @default.
- W1968950287 cites W1577776424 @default.
- W1968950287 cites W1945832201 @default.
- W1968950287 cites W1965963242 @default.
- W1968950287 cites W1968093456 @default.
- W1968950287 cites W1969557235 @default.
- W1968950287 cites W1973852009 @default.
- W1968950287 cites W1976319973 @default.
- W1968950287 cites W1986087667 @default.
- W1968950287 cites W1991548299 @default.
- W1968950287 cites W2003992614 @default.
- W1968950287 cites W2013452232 @default.
- W1968950287 cites W2016839351 @default.
- W1968950287 cites W2023781876 @default.
- W1968950287 cites W2024040954 @default.
- W1968950287 cites W2026018179 @default.
- W1968950287 cites W2040198458 @default.
- W1968950287 cites W2062007861 @default.
- W1968950287 cites W2063513614 @default.
- W1968950287 cites W2068295458 @default.
- W1968950287 cites W2068818952 @default.
- W1968950287 cites W2082559281 @default.
- W1968950287 cites W2088590824 @default.
- W1968950287 cites W2090801567 @default.
- W1968950287 cites W2092964019 @default.
- W1968950287 cites W2114164301 @default.
- W1968950287 cites W2115800876 @default.
- W1968950287 cites W2116949924 @default.
- W1968950287 cites W2132075043 @default.
- W1968950287 cites W2151887581 @default.
- W1968950287 cites W2171094619 @default.
- W1968950287 cites W2020382075 @default.
- W1968950287 doi "https://doi.org/10.1002/jbmr.5650110505" @default.
- W1968950287 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9157772" @default.
- W1968950287 hasPublicationYear "2009" @default.
- W1968950287 type Work @default.
- W1968950287 sameAs 1968950287 @default.
- W1968950287 citedByCount "42" @default.
- W1968950287 countsByYear W19689502872016 @default.
- W1968950287 countsByYear W19689502872018 @default.
- W1968950287 countsByYear W19689502872020 @default.
- W1968950287 countsByYear W19689502872023 @default.
- W1968950287 crossrefType "journal-article" @default.
- W1968950287 hasAuthorship W1968950287A5029762083 @default.
- W1968950287 hasAuthorship W1968950287A5038619650 @default.
- W1968950287 hasAuthorship W1968950287A5061689840 @default.
- W1968950287 hasAuthorship W1968950287A5067422009 @default.
- W1968950287 hasAuthorship W1968950287A5072904368 @default.
- W1968950287 hasConcept C11960822 @default.
- W1968950287 hasConcept C135285700 @default.
- W1968950287 hasConcept C144174609 @default.
- W1968950287 hasConcept C153911025 @default.
- W1968950287 hasConcept C170493617 @default.
- W1968950287 hasConcept C17971392 @default.
- W1968950287 hasConcept C185592680 @default.
- W1968950287 hasConcept C2775960820 @default.
- W1968950287 hasConcept C2776414213 @default.
- W1968950287 hasConcept C2778938600 @default.
- W1968950287 hasConcept C33235085 @default.
- W1968950287 hasConcept C55493867 @default.
- W1968950287 hasConcept C56165258 @default.
- W1968950287 hasConcept C75184817 @default.
- W1968950287 hasConcept C78976303 @default.
- W1968950287 hasConcept C86803240 @default.
- W1968950287 hasConcept C92759361 @default.
- W1968950287 hasConceptScore W1968950287C11960822 @default.
- W1968950287 hasConceptScore W1968950287C135285700 @default.
- W1968950287 hasConceptScore W1968950287C144174609 @default.
- W1968950287 hasConceptScore W1968950287C153911025 @default.
- W1968950287 hasConceptScore W1968950287C170493617 @default.
- W1968950287 hasConceptScore W1968950287C17971392 @default.
- W1968950287 hasConceptScore W1968950287C185592680 @default.
- W1968950287 hasConceptScore W1968950287C2775960820 @default.
- W1968950287 hasConceptScore W1968950287C2776414213 @default.
- W1968950287 hasConceptScore W1968950287C2778938600 @default.
- W1968950287 hasConceptScore W1968950287C33235085 @default.
- W1968950287 hasConceptScore W1968950287C55493867 @default.
- W1968950287 hasConceptScore W1968950287C56165258 @default.
- W1968950287 hasConceptScore W1968950287C75184817 @default.
- W1968950287 hasConceptScore W1968950287C78976303 @default.
- W1968950287 hasConceptScore W1968950287C86803240 @default.
- W1968950287 hasConceptScore W1968950287C92759361 @default.
- W1968950287 hasIssue "5" @default.
- W1968950287 hasLocation W19689502871 @default.
- W1968950287 hasLocation W19689502872 @default.