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• W1969018052 abstract "A method which combines fluorescence spectroscopy, guest–host chemistry, and principal component regression (PCR) was proposed for determining the enantiomeric composition of tryptophan (Trp). Fluorescence spectroscopy was used to measure the chiral interaction between the enantiomers of tryptophan and bovine serum albumin (BSA), which plays the role of chiral selector. PCR was used to model the quantitative relationship between the obtained fluorescence spectral data and enantiomeric composition of Trp. Leave-one-out cross validation and external test validation were employed to validate the proposed method. The results demonstrate that the method is practical in quantitatively determining enantiomeric composition of Trp. Moreover, the method shows high sensitivity. When there was 2.50 µmol · L−1 Trp in the solutions, the enantiomeric composition of tryptophan was accurately determined." @default.
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• W1969018052 date "2013-03-24" @default.
• W1969018052 modified "2023-09-26" @default.
• W1969018052 title "Determination of Enantiomeric Composition of Tryptophan by Fluorescence Spectroscopy Combined with Principal Component Regression" @default.
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• W1969018052 doi "https://doi.org/10.1080/00032719.2012.733901" @default.
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