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• W1969041636 abstract "The effect of divalent cations on the primary photoconversion kinetics of chloroplast Photosystems (PS) I and II was investigated by absorbance difference spectrophotometry in the ultraviolet (ΔA320) and red (ΔA700) regions and by fluorescence at room temperature. Three main chlorophyll (Chl) a fluorescence emission components were identified. Addition of 5 mM MgCl2 to unstacked chloroplasts caused a 5–7-fold increase in Fvα, the variable fluorescence yield controlled by the α-centers. The fluorescence yield Fvβ controlled by the β-centers and the nonvariable fluorescence yield F0 were only slightly changed by the treatment. The absolute number of α- and β-centers remained unchanged and independent of divalent cations. The rate constants Kα, Kβ and KP-700 determined from the photoconversion kinetics of Qα, Qβ and P-700 were also unchanged by divalent cations, suggesting a constancy of the respective absorption cross-sections. Evidence is presented that the Mg2+ effect on Chl a fluorescence is not due simply to unstacking. Conclusion: (1) In the absence of divalent cations from the chloroplast suspending medium, the variable fluorescence yield is not complementary to the rate of PS II photochemistry. (2) A spillover of excitation from PS II to PS I in the absence of Mg2+ cannot account for the 7-fold lowering of the variable fluorescence yield Fvα at room temperature. The results are discussed in view of a model of excitation transfer and fluorescence emission in the pigment bed of PS IIα and PS IIβ." @default.
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• W1969041636 creator A5078308526 @default.
• W1969041636 date "1982-10-01" @default.
• W1969041636 modified "2023-09-24" @default.
• W1969041636 title "Photoconversion kinetics of chloroplast Photosystems I and II. Effect of Mg2+" @default.
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• W1969041636 doi "https://doi.org/10.1016/0005-2728(82)90113-x" @default.
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