FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1969048905> ?p ?o ?g. }

• W1969048905 endingPage "9015" @default.
• W1969048905 startingPage "9008" @default.
• W1969048905 abstract "A functional interaction between DNA helicase E and DNA polymerase epsilon from calf thymus has been detected which results in the extension of an upstream 3' OH through a downstream primer to the end of a synthetic template. DNA synthesis resulting in full-length extension products was dependent on the addition of DNA helicase E and hydrolysis of ATP, suggesting that displacement of the downstream primer was required. Identical reactions using DNA polymerases alpha and delta in place of DNA polymerase epsilon showed no full-length products dependent on helicase E, indicating that polymerases alpha and delta were incapable of functionally interacting with the helicase. The reaction leading to full-length extension products was time dependent and dependent on the concentration of added polymerase epsilon and helicase E. Exonucleolytic degradation of the downstream primer, or ligation of the downstream primer to the upstream 3' OH, were not responsible for the full-length products observed. Displacement of the downstream primer by DNA helicase E was not affected by the addition of polymerase epsilon to the reactions. Template dilution experiments demonstrated that DNA polymerase epsilon and helicase E were acting in concert to perform displacement synthesis. Additional evidence for functional coordination was obtained by demonstration that DNA helicase E stimulated DNA polymerase epsilon in a standard DNA synthetic assay using dA3000.dT16 as the template-primer. The results presented are consistent with the hypothesis that DNA helicase E and DNA polymerase epsilon are capable of coordinated activities that result in displacement synthesis. A functional interaction of this sort may be involved at the eukaryotic replication fork or in DNA repair." @default.
• W1969048905 created "2016-06-24" @default.
• W1969048905 creator A5005315226 @default.
• W1969048905 creator A5005809670 @default.
• W1969048905 creator A5029345329 @default.
• W1969048905 date "1992-09-22" @default.
• W1969048905 modified "2023-10-18" @default.
• W1969048905 title "DNA helicase E and DNA polymerase .epsilon. functionally interact for displacement synthesis" @default.
• W1969048905 cites W1485697008 @default.
• W1969048905 cites W1487346902 @default.
• W1969048905 cites W1499280348 @default.
• W1969048905 cites W1525007860 @default.
• W1969048905 cites W1525394119 @default.
• W1969048905 cites W1528139698 @default.
• W1969048905 cites W1529710337 @default.
• W1969048905 cites W1535892176 @default.
• W1969048905 cites W1540815504 @default.
• W1969048905 cites W1552394997 @default.
• W1969048905 cites W1560152642 @default.
• W1969048905 cites W1574197136 @default.
• W1969048905 cites W1594126558 @default.
• W1969048905 cites W1604233325 @default.
• W1969048905 cites W1608564947 @default.
• W1969048905 cites W1657088788 @default.
• W1969048905 cites W1846876036 @default.
• W1969048905 cites W1945304539 @default.
• W1969048905 cites W1966340370 @default.
• W1969048905 cites W1974330331 @default.
• W1969048905 cites W1989075626 @default.
• W1969048905 cites W1991548363 @default.
• W1969048905 cites W1998604681 @default.
• W1969048905 cites W2014450282 @default.
• W1969048905 cites W2016327026 @default.
• W1969048905 cites W2024521512 @default.
• W1969048905 cites W2028527119 @default.
• W1969048905 cites W2033283534 @default.
• W1969048905 cites W2058854434 @default.
• W1969048905 cites W2067752707 @default.
• W1969048905 cites W206882731 @default.
• W1969048905 cites W2073499220 @default.
• W1969048905 cites W2081184332 @default.
• W1969048905 cites W2092791914 @default.
• W1969048905 cites W2094230374 @default.
• W1969048905 cites W2123846442 @default.
• W1969048905 cites W2136238750 @default.
• W1969048905 cites W2136423582 @default.
• W1969048905 cites W2139768549 @default.
• W1969048905 cites W2169154093 @default.
• W1969048905 doi "https://doi.org/10.1021/bi00152a043" @default.
• W1969048905 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1327108" @default.
• W1969048905 hasPublicationYear "1992" @default.
• W1969048905 type Work @default.
• W1969048905 sameAs 1969048905 @default.
• W1969048905 citedByCount "12" @default.
• W1969048905 crossrefType "journal-article" @default.
• W1969048905 hasAuthorship W1969048905A5005315226 @default.
• W1969048905 hasAuthorship W1969048905A5005809670 @default.
• W1969048905 hasAuthorship W1969048905A5029345329 @default.
• W1969048905 hasConcept C104317684 @default.
• W1969048905 hasConcept C116988904 @default.
• W1969048905 hasConcept C153911025 @default.
• W1969048905 hasConcept C156719811 @default.
• W1969048905 hasConcept C161223559 @default.
• W1969048905 hasConcept C164965036 @default.
• W1969048905 hasConcept C178790620 @default.
• W1969048905 hasConcept C185592680 @default.
• W1969048905 hasConcept C194830204 @default.
• W1969048905 hasConcept C2756471 @default.
• W1969048905 hasConcept C2777563447 @default.
• W1969048905 hasConcept C31254050 @default.
• W1969048905 hasConcept C49105822 @default.
• W1969048905 hasConcept C552990157 @default.
• W1969048905 hasConcept C55493867 @default.
• W1969048905 hasConcept C58167604 @default.
• W1969048905 hasConcept C64241487 @default.
• W1969048905 hasConcept C66072924 @default.
• W1969048905 hasConcept C67705224 @default.
• W1969048905 hasConcept C82381507 @default.
• W1969048905 hasConcept C86803240 @default.
• W1969048905 hasConcept C88354546 @default.
• W1969048905 hasConceptScore W1969048905C104317684 @default.
• W1969048905 hasConceptScore W1969048905C116988904 @default.
• W1969048905 hasConceptScore W1969048905C153911025 @default.
• W1969048905 hasConceptScore W1969048905C156719811 @default.
• W1969048905 hasConceptScore W1969048905C161223559 @default.
• W1969048905 hasConceptScore W1969048905C164965036 @default.
• W1969048905 hasConceptScore W1969048905C178790620 @default.
• W1969048905 hasConceptScore W1969048905C185592680 @default.
• W1969048905 hasConceptScore W1969048905C194830204 @default.
• W1969048905 hasConceptScore W1969048905C2756471 @default.
• W1969048905 hasConceptScore W1969048905C2777563447 @default.
• W1969048905 hasConceptScore W1969048905C31254050 @default.
• W1969048905 hasConceptScore W1969048905C49105822 @default.
• W1969048905 hasConceptScore W1969048905C552990157 @default.
• W1969048905 hasConceptScore W1969048905C55493867 @default.
• W1969048905 hasConceptScore W1969048905C58167604 @default.
• W1969048905 hasConceptScore W1969048905C64241487 @default.
• W1969048905 hasConceptScore W1969048905C66072924 @default.

• next