FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1969101538> ?p ?o ?g. }

• W1969101538 endingPage "745" @default.
• W1969101538 startingPage "739" @default.
• W1969101538 abstract "The matrix metalloproteinase (MMP)-2 has a crucial role in extracellular matrix degradation associated with cancer metastasis and angiogenesis. The latent form, pro-MMP-2, is activated on the cell surface by the membrane-tethered membrane type 1 (MT1)-MMP, in a process regulated by the tissue inhibitor of metalloproteinase (TIMP)-2. A complex of active MT1-MMP and TIMP-2 binds pro-MMP-2 forming a ternary complex, which permits pro-MMP-2 activation by a TIMP-2-free neighbouring MT1-MMP. It remains unclear how MMP-2 activity in the pericellular space is regulated in the presence of TIMP-2. To address this question, the effect of TIMP-2 on MMP-2 activity in the extracellular space was investigated in live cells, and their isolated plasma membrane fractions, engineered to control the relative levels of MT1-MMP and TIMP-2 expression. We show that both free and inhibited MMP-2 is detected in the medium, and that the net MMP-2 activity correlates with the level of TIMP-2 expression. Studies to displace MT1-MMP-bound TIMP-2 in a purified system with active MMP-2 show minimal displacement of inhibitor, under the experimental conditions, due to the high affinity interaction between TIMP-2 and MT1-MMP. Thus inhibition of MMP-2 activity in the extracellular space is unlikely to result solely as a result of TIMP-2 dissociation from its complex with MT1-MMP. Consistently, immunoblot analyses of plasma membranes, and surface biotinylation experiments show that the level of surface association of TIMP-2 is independent of MT1-MMP expression. Thus low-affinity binding of TIMP-2 to sites distinct to MT1-MMP may have a role in regulating MMP-2 activity in the extracellular space generated by the ternary complex." @default.
• W1969101538 created "2016-06-24" @default.
• W1969101538 creator A5005322119 @default.
• W1969101538 creator A5063462261 @default.
• W1969101538 date "2003-09-15" @default.
• W1969101538 modified "2023-09-25" @default.
• W1969101538 title "TIMP-2 (tissue inhibitor of metalloproteinase-2) regulates MMP-2 (matrix metalloproteinase-2) activity in the extracellular environment after pro-MMP-2 activation by MT1 (membrane type 1)-MMP" @default.
• W1969101538 cites W1488746545 @default.
• W1969101538 cites W1501988955 @default.
• W1969101538 cites W1619454078 @default.
• W1969101538 cites W1700189391 @default.
• W1969101538 cites W1943421922 @default.
• W1969101538 cites W1964855331 @default.
• W1969101538 cites W1971343950 @default.
• W1969101538 cites W1978309686 @default.
• W1969101538 cites W1979390430 @default.
• W1969101538 cites W1998606357 @default.
• W1969101538 cites W2004101410 @default.
• W1969101538 cites W2004307784 @default.
• W1969101538 cites W2022065340 @default.
• W1969101538 cites W2048996380 @default.
• W1969101538 cites W2049220062 @default.
• W1969101538 cites W2051991758 @default.
• W1969101538 cites W2056420432 @default.
• W1969101538 cites W2063960068 @default.
• W1969101538 cites W2064852156 @default.
• W1969101538 cites W2068843202 @default.
• W1969101538 cites W2070004726 @default.
• W1969101538 cites W2074005418 @default.
• W1969101538 cites W2076718353 @default.
• W1969101538 cites W2083533326 @default.
• W1969101538 cites W2087298204 @default.
• W1969101538 cites W2088172329 @default.
• W1969101538 cites W2091009027 @default.
• W1969101538 cites W2092449976 @default.
• W1969101538 cites W2092648346 @default.
• W1969101538 cites W2121492332 @default.
• W1969101538 cites W2141830788 @default.
• W1969101538 cites W2152247362 @default.
• W1969101538 cites W2156234950 @default.
• W1969101538 cites W2188357563 @default.
• W1969101538 cites W41086465 @default.
• W1969101538 cites W2123876676 @default.
• W1969101538 doi "https://doi.org/10.1042/bj20030557" @default.
• W1969101538 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1223627" @default.
• W1969101538 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12755684" @default.
• W1969101538 hasPublicationYear "2003" @default.
• W1969101538 type Work @default.
• W1969101538 sameAs 1969101538 @default.
• W1969101538 citedByCount "159" @default.
• W1969101538 countsByYear W19691015382012 @default.
• W1969101538 countsByYear W19691015382013 @default.
• W1969101538 countsByYear W19691015382014 @default.
• W1969101538 countsByYear W19691015382015 @default.
• W1969101538 countsByYear W19691015382016 @default.
• W1969101538 countsByYear W19691015382017 @default.
• W1969101538 countsByYear W19691015382018 @default.
• W1969101538 countsByYear W19691015382019 @default.
• W1969101538 countsByYear W19691015382020 @default.
• W1969101538 countsByYear W19691015382021 @default.
• W1969101538 countsByYear W19691015382022 @default.
• W1969101538 countsByYear W19691015382023 @default.
• W1969101538 crossrefType "journal-article" @default.
• W1969101538 hasAuthorship W1969101538A5005322119 @default.
• W1969101538 hasAuthorship W1969101538A5063462261 @default.
• W1969101538 hasBestOaLocation W19691015382 @default.
• W1969101538 hasConcept C109523444 @default.
• W1969101538 hasConcept C181199279 @default.
• W1969101538 hasConcept C185592680 @default.
• W1969101538 hasConcept C189165786 @default.
• W1969101538 hasConcept C2775958869 @default.
• W1969101538 hasConcept C2775965246 @default.
• W1969101538 hasConcept C2776223131 @default.
• W1969101538 hasConcept C2780394083 @default.
• W1969101538 hasConcept C28406088 @default.
• W1969101538 hasConcept C502942594 @default.
• W1969101538 hasConcept C55493867 @default.
• W1969101538 hasConcept C55728118 @default.
• W1969101538 hasConcept C86756495 @default.
• W1969101538 hasConcept C86803240 @default.
• W1969101538 hasConcept C95444343 @default.
• W1969101538 hasConceptScore W1969101538C109523444 @default.
• W1969101538 hasConceptScore W1969101538C181199279 @default.
• W1969101538 hasConceptScore W1969101538C185592680 @default.
• W1969101538 hasConceptScore W1969101538C189165786 @default.
• W1969101538 hasConceptScore W1969101538C2775958869 @default.
• W1969101538 hasConceptScore W1969101538C2775965246 @default.
• W1969101538 hasConceptScore W1969101538C2776223131 @default.
• W1969101538 hasConceptScore W1969101538C2780394083 @default.
• W1969101538 hasConceptScore W1969101538C28406088 @default.
• W1969101538 hasConceptScore W1969101538C502942594 @default.
• W1969101538 hasConceptScore W1969101538C55493867 @default.
• W1969101538 hasConceptScore W1969101538C55728118 @default.
• W1969101538 hasConceptScore W1969101538C86756495 @default.
• W1969101538 hasConceptScore W1969101538C86803240 @default.
• W1969101538 hasConceptScore W1969101538C95444343 @default.
• W1969101538 hasIssue "3" @default.
• W1969101538 hasLocation W19691015381 @default.

• next