Matches in SemOpenAlex for { <https://semopenalex.org/work/W1969121607> ?p ?o ?g. }
- W1969121607 endingPage "12454" @default.
- W1969121607 startingPage "12446" @default.
- W1969121607 abstract "There is considerable uncertainty about the precise secondary structure adopted by the M13 coat protein when embedded in a phospholipid bilayer. Circular dichroism (CD) spectroscopy suggests that a major change in the structure of the coat protein occurs upon membrane insertion. It is reported that the structure of the protein in the membrane has only about 50% alpha-helix, the rest being mainly in a beta-sheet conformation, whereas the protein is almost completely alpha-helical when intact in the phage. In this study we have undertaken a spectroscopic analysis using Fourier transform infrared, Raman, and CD spectroscopy to characterize the secondary structure of M13 coat protein when present in membranes consisting of dioleoylphosphatidylglycerol and dimyristoylphosphatidylglycerol. In sharp contrast to earlier CD studies, our results indicate that the coat protein in its membrane-embedded state has a very high alpha-helical content with virtually no beta-sheet structures present. This result indicates that the structures of the coat protein when intact in the phage or when embedded in the membrane are similar. Although our results differ from earlier CD studies, they are consistent with a recent NMR study, which showed that the M13 coat protein in sodium dodecyl sulfate micelles is primarily alpha-helical with no evidence for beta-sheet structure [Henry, G. D., & Sykes, B.D. (1992) Biochemistry 31, 5284-5297]. These results lead to the conclusion that the M13 coat protein can insert from the membrane-bound state into a virus particle with a similar secondary structure, without large energy implications.(ABSTRACT TRUNCATED AT 250 WORDS)" @default.
- W1969121607 created "2016-06-24" @default.
- W1969121607 creator A5020176415 @default.
- W1969121607 creator A5034888541 @default.
- W1969121607 creator A5045872090 @default.
- W1969121607 creator A5056350473 @default.
- W1969121607 creator A5077593543 @default.
- W1969121607 date "1993-11-01" @default.
- W1969121607 modified "2023-10-10" @default.
- W1969121607 title "Secondary structure of M13 coat protein in phospholipids studied by circular dichroism, Raman, and Fourier transform infrared spectroscopy" @default.
- W1969121607 cites W1510061653 @default.
- W1969121607 cites W1529097157 @default.
- W1969121607 cites W1547429701 @default.
- W1969121607 cites W1563125773 @default.
- W1969121607 cites W1584375360 @default.
- W1969121607 cites W1584921250 @default.
- W1969121607 cites W1969457124 @default.
- W1969121607 cites W1974917255 @default.
- W1969121607 cites W1975046921 @default.
- W1969121607 cites W1975608923 @default.
- W1969121607 cites W1978690762 @default.
- W1969121607 cites W1982133298 @default.
- W1969121607 cites W1984435444 @default.
- W1969121607 cites W1985387785 @default.
- W1969121607 cites W1985643908 @default.
- W1969121607 cites W1986228145 @default.
- W1969121607 cites W1987205935 @default.
- W1969121607 cites W1992453230 @default.
- W1969121607 cites W2001553566 @default.
- W1969121607 cites W2005381117 @default.
- W1969121607 cites W2014349016 @default.
- W1969121607 cites W2014468914 @default.
- W1969121607 cites W2014550271 @default.
- W1969121607 cites W2022226258 @default.
- W1969121607 cites W2028889403 @default.
- W1969121607 cites W2035570438 @default.
- W1969121607 cites W2035743228 @default.
- W1969121607 cites W2039324314 @default.
- W1969121607 cites W2041374005 @default.
- W1969121607 cites W2041959385 @default.
- W1969121607 cites W2042244352 @default.
- W1969121607 cites W2044208863 @default.
- W1969121607 cites W2051788505 @default.
- W1969121607 cites W2055601862 @default.
- W1969121607 cites W2067637442 @default.
- W1969121607 cites W2067807705 @default.
- W1969121607 cites W2069258864 @default.
- W1969121607 cites W2073039991 @default.
- W1969121607 cites W2073531236 @default.
- W1969121607 cites W2078798721 @default.
- W1969121607 cites W2081279009 @default.
- W1969121607 cites W2082389960 @default.
- W1969121607 cites W2083276659 @default.
- W1969121607 cites W2083457891 @default.
- W1969121607 cites W2087116150 @default.
- W1969121607 cites W2087501877 @default.
- W1969121607 cites W2088107416 @default.
- W1969121607 cites W2094077114 @default.
- W1969121607 cites W2094331619 @default.
- W1969121607 cites W2113972334 @default.
- W1969121607 cites W2129416952 @default.
- W1969121607 cites W2136087328 @default.
- W1969121607 cites W2153994334 @default.
- W1969121607 cites W2159964891 @default.
- W1969121607 cites W2160217048 @default.
- W1969121607 cites W2167139807 @default.
- W1969121607 cites W2167596401 @default.
- W1969121607 cites W2169432471 @default.
- W1969121607 cites W2391154686 @default.
- W1969121607 cites W2401376648 @default.
- W1969121607 cites W4247051568 @default.
- W1969121607 doi "https://doi.org/10.1021/bi00097a024" @default.
- W1969121607 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8241135" @default.
- W1969121607 hasPublicationYear "1993" @default.
- W1969121607 type Work @default.
- W1969121607 sameAs 1969121607 @default.
- W1969121607 citedByCount "36" @default.
- W1969121607 countsByYear W19691216072013 @default.
- W1969121607 countsByYear W19691216072017 @default.
- W1969121607 countsByYear W19691216072019 @default.
- W1969121607 crossrefType "journal-article" @default.
- W1969121607 hasAuthorship W1969121607A5020176415 @default.
- W1969121607 hasAuthorship W1969121607A5034888541 @default.
- W1969121607 hasAuthorship W1969121607A5045872090 @default.
- W1969121607 hasAuthorship W1969121607A5056350473 @default.
- W1969121607 hasAuthorship W1969121607A5077593543 @default.
- W1969121607 hasConcept C121332964 @default.
- W1969121607 hasConcept C12554922 @default.
- W1969121607 hasConcept C133571119 @default.
- W1969121607 hasConcept C159547669 @default.
- W1969121607 hasConcept C185592680 @default.
- W1969121607 hasConcept C18903297 @default.
- W1969121607 hasConcept C2778530040 @default.
- W1969121607 hasConcept C2778918659 @default.
- W1969121607 hasConcept C2779965526 @default.
- W1969121607 hasConcept C32891209 @default.
- W1969121607 hasConcept C39944091 @default.
- W1969121607 hasConcept C41625074 @default.