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- W1969148927 abstract "The goal of this study was to evaluate the potential suitability of collagen Vitrigel (CV) membrane as a substrate for the separate reconstruction of the three main cellular layers of the cornea. Limbal explants, keratocytes, and endothelial cells were cultured on transparent membranes made of type I collagen. The resulting cell sheets were evaluated using RT-PCR, in addition to light and electron microscopy. Tensile testing was also performed to examine the mechanical properties of CV. Limbal explant cultures resulted in partially stratified epithelial sheets with upregulation of the putative stem cell marker p63. Keratocytes cultured in serum on CV exhibited stellate morphology along with a marked increase in expression of corneal crystallin ALDH and keratocan, (a keratan sulphate proteoglycan: KSPG), compared to identical cultures on tissue culture plastic. Endothelial cells formed dense monolayers with uniform cell size, tight intercellular junctions, and expression of voltage-dependent anion channels VDAC2 and VDAC3, chloride channel protein CLCN2, and sodium bicarbonate transporter NBC1. Epithelial and endothelial cells exhibited adhesive structures (desmosomes and hemidesmosomes) and evidence of apical specialization (microplicae), while endothelial cells also produced a Descemet's membrane-like basal lamina. CV was found to possess ultimate tensile strengths of 6.8 +/- 1.5 MPa when hydrated and 28.6 +/- 7.0 MPa when dry. Taken together, these results indicate that CV holds promise as a substrate for corneal reconstruction." @default.
- W1969148927 created "2016-06-24" @default.
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- W1969148927 date "2009-03-12" @default.
- W1969148927 modified "2023-10-18" @default.
- W1969148927 title "Collagen vitrigel membranes for the<i>in vitro</i>reconstruction of separate corneal epithelial, stromal, and endothelial cell layers" @default.
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- W1969148927 cites W1589803235 @default.
- W1969148927 cites W160357853 @default.
- W1969148927 cites W1965083225 @default.
- W1969148927 cites W1965941046 @default.
- W1969148927 cites W1976130708 @default.
- W1969148927 cites W1976468302 @default.
- W1969148927 cites W1978227510 @default.
- W1969148927 cites W1979015651 @default.
- W1969148927 cites W1981013532 @default.
- W1969148927 cites W1986085958 @default.
- W1969148927 cites W1988354512 @default.
- W1969148927 cites W1991112222 @default.
- W1969148927 cites W1997505481 @default.
- W1969148927 cites W1998443601 @default.
- W1969148927 cites W2000066348 @default.
- W1969148927 cites W2002844989 @default.
- W1969148927 cites W2004016543 @default.
- W1969148927 cites W2008737688 @default.
- W1969148927 cites W2009101760 @default.
- W1969148927 cites W2013135605 @default.
- W1969148927 cites W2013679917 @default.
- W1969148927 cites W2019842452 @default.
- W1969148927 cites W2020722287 @default.
- W1969148927 cites W2021490900 @default.
- W1969148927 cites W2023451731 @default.
- W1969148927 cites W2026545622 @default.
- W1969148927 cites W2027658103 @default.
- W1969148927 cites W2028237347 @default.
- W1969148927 cites W2031151230 @default.
- W1969148927 cites W2031721300 @default.
- W1969148927 cites W2035443956 @default.
- W1969148927 cites W2036607348 @default.
- W1969148927 cites W2037455401 @default.
- W1969148927 cites W2039157833 @default.
- W1969148927 cites W2039318866 @default.
- W1969148927 cites W2040450375 @default.
- W1969148927 cites W2042216373 @default.
- W1969148927 cites W2043541388 @default.
- W1969148927 cites W2046914886 @default.
- W1969148927 cites W2049517167 @default.
- W1969148927 cites W2054152044 @default.
- W1969148927 cites W2056796251 @default.
- W1969148927 cites W2057364537 @default.
- W1969148927 cites W2057796209 @default.
- W1969148927 cites W2065624075 @default.
- W1969148927 cites W2068195795 @default.
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- W1969148927 cites W2083082681 @default.
- W1969148927 cites W2089858022 @default.
- W1969148927 cites W2093583163 @default.
- W1969148927 cites W2096604029 @default.
- W1969148927 cites W2101549626 @default.
- W1969148927 cites W2114425621 @default.
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- W1969148927 cites W2119261695 @default.
- W1969148927 cites W2123630792 @default.
- W1969148927 cites W2127033380 @default.
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- W1969148927 cites W2330446371 @default.
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- W1969148927 doi "https://doi.org/10.1002/jbm.b.31351" @default.
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