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• W1969182483 abstract "Droplet-based microfluidics has raised a lot of interest recently due to its wide applications to screening biological/chemical assays with high throughput. Despite the advances on droplet-based assays involving cells, gene delivery methods that are compatible with the droplet platform have been lacking. In this report, we demonstrate a simple microfluidic device that encapsulates cells into aqueous droplets and then electroporates the encapsulated cells. The electroporation occurs when the cell-containing droplets (in oil) flow through a pair of microelectrodes with a constant voltage established in between. We investigate the parameters and characteristics of the electroporation. We demonstrate delivering enhanced green fluorescent protein (EGFP) plasmid into Chinese hamster ovary (CHO) cells. We envision the application of this technique to high-throughput functional genomics studies based on droplet microfluidics." @default.
• W1969182483 created "2016-06-24" @default.
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• W1969182483 date "2009-02-06" @default.
• W1969182483 modified "2023-10-12" @default.
• W1969182483 title "Electroporation of Cells in Microfluidic Droplets" @default.
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• W1969182483 doi "https://doi.org/10.1021/ac9001172" @default.
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