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- W1969262020 abstract "The poly(hexamethylenebiguanidinium chloride) [PHMBH + Cl−] assay method developed originally for sodium alginate determination in solution (0 01-0 5 %) has been applied to a wide range of other acidic polysaccharides. The simple procedure consists of the addition of a known excess of PHMBH + Cl− to precipitate the sample quantitatively and thereafter measuring the u.v. absorption of residual PHMBH + Cl− in the supernatant. Response to the method is dependent upon the acidic group-monosaccharide molar ratio and can be conveniently altered for polysaccharides with different anionic charge densities by simple adjustment of assay conditions. For acidic polysaccharides with very low charge densities, such as propylene glycol alginates with a high degree of esterification, the response to the assay can be increased by addition of borate ions which complex with the polymer molecules to increase the negative charge. The electrostatic nature of the response also allows the assay method to be used for the determination of extent of esterification in propylene glycol alginates with up to ∼70% derivatisation. The method has been shown to be suitable for low molecular weight samples. Therefore, it is more reliable than other assay methods for acidic polysaccharides which involve a precipitation step with doubtful quantitative recovery for low molecular weight material. The simplicity, rapidity and reliability of the method makes it a suitable procedure for both carboxylated and sulphated polysaccharides in a range of applications." @default.
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- W1969262020 date "1987-09-01" @default.
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- W1969262020 title "Poiy(hexamethyienebigyanidiniym chloride) as a reagent for the determination of water-soluble naturai carboxylate and sulphate polymers" @default.
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- W1969262020 doi "https://doi.org/10.1002/pi.4980190504" @default.
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