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- W1969279681 abstract "An assay procedure utilizing pulsed amperometric detection at a platinum-particles modified electrode has been developed for the determination of cysteine and glutathione in blood samples following preliminary separation by reversed-phase liquid chromatography. A chemically modified electrode (CME) constructed by unique electroreduction from a platinum-salt solution to produce dispersed Pt particles on a glassy carbon surface was demonstrated to catalyze the electo-oxidation of sulfhydryl-containing compounds: dl-cysteine (CYS), reduced glutathione (GSH). When used as the sensing electrode in flow-system pulsed-amperometric detection (PAD), electrode fouling could be avoided using a waveform in which the cathodic reactivation process occurred at a potential of −1.0 V vs. Ag/AgCl to achieve a cathodic desorption of atomic sulfur. A superior detection limit for these free thiols was obtained at a Pt particle-based GC electrode compared with other methods; this novel dispersed Pt particles CME exhibited high electrocatalytic stability and activity when it was employed as an electrochemical detector in FIA and HPLC for the determination of those organo-sulfur compounds." @default.
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- W1969279681 date "1995-08-01" @default.
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- W1969279681 title "Determination of Cysteine and Reduced Glutathione in Human Plasma by Liquid Chromatography with Pulsed Amperometric Electrochemical Detection Using a Platinum-Particles Modified Glassy Carbon Electrode" @default.
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- W1969279681 doi "https://doi.org/10.2116/analsci.11.597" @default.
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