FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1969331397> ?p ?o ?g. }

• W1969331397 endingPage "251" @default.
• W1969331397 startingPage "243" @default.
• W1969331397 abstract "The DNA-dependent protein kinase (DNA-PK) is required for the repair of DNA double-strand breaks (DSBs), such as those caused by ionizing radiation and other DNA-damaging agents. DNA-PK is composed of a large catalytic subunit (DNA-PKcs) and a heterodimer of Ku70 and Ku80 that assemble on the ends of double-stranded DNA to form an active serine/threonine protein kinase complex. Despite in vitro and in vivo evidence to support an essential role for the protein kinase activity of DNA-PK in the repair of DNA DSBs, the physiological targets of DNA-PK have remained elusive. We have previously shown that DNA-PK undergoes autophosphorylation in vitro, and that autophosphorylation correlates with loss of protein kinase activity and dissociation of the DNA-PK complex. Also, treatment of cells with the protein phosphatase inhibitor, okadaic acid, enhances DNA-PKcs phosphorylation and reduces DNA-PK activity in vivo. Here, using solid-phase protein sequencing, MS and phosphospecific antibodies, we have identified seven in vitro autophosphorylation sites in DNA-PKcs. Six of these sites (Thr2609, Ser2612, Thr2620, Ser2624, Thr2638 and Thr2647) are clustered in a region of 38 amino acids in the central region of the protein. Five of these sites (Thr2609, Ser2612, Thr2638, Thr2647 and Ser3205) are conserved between six vertebrate species. Moreover, we show that DNA-PKcs is phosphorylated in vivo at Thr2609, Ser2612, Thr2638 and Thr2647 in okadaic acid-treated human cells. We propose that phosphorylation of these sites may play an important role in DNA-PK function." @default.
• W1969331397 created "2016-06-24" @default.
• W1969331397 creator A5020583214 @default.
• W1969331397 creator A5028009861 @default.
• W1969331397 creator A5029706709 @default.
• W1969331397 creator A5035430209 @default.
• W1969331397 creator A5035453779 @default.
• W1969331397 creator A5041748356 @default.
• W1969331397 creator A5054181982 @default.
• W1969331397 creator A5079162936 @default.
• W1969331397 creator A5091310822 @default.
• W1969331397 date "2002-11-15" @default.
• W1969331397 modified "2023-10-14" @default.
• W1969331397 title "Identification of in vitro and in vivo phosphorylation sites in the catalytic subunit of the DNA-dependent protein kinase" @default.
• W1969331397 cites W1499226315 @default.
• W1969331397 cites W1899395269 @default.
• W1969331397 cites W1907438453 @default.
• W1969331397 cites W1959049775 @default.
• W1969331397 cites W1967780354 @default.
• W1969331397 cites W1972313386 @default.
• W1969331397 cites W1974010399 @default.
• W1969331397 cites W1977145510 @default.
• W1969331397 cites W1979303367 @default.
• W1969331397 cites W1986448084 @default.
• W1969331397 cites W1997354716 @default.
• W1969331397 cites W2004370917 @default.
• W1969331397 cites W2011522975 @default.
• W1969331397 cites W2016552344 @default.
• W1969331397 cites W2016799564 @default.
• W1969331397 cites W2025987815 @default.
• W1969331397 cites W2031365072 @default.
• W1969331397 cites W2043431835 @default.
• W1969331397 cites W2044874191 @default.
• W1969331397 cites W2046592596 @default.
• W1969331397 cites W2059823430 @default.
• W1969331397 cites W2061337059 @default.
• W1969331397 cites W2065022250 @default.
• W1969331397 cites W2077727614 @default.
• W1969331397 cites W2082910780 @default.
• W1969331397 cites W2087343934 @default.
• W1969331397 cites W2089574530 @default.
• W1969331397 cites W2091625765 @default.
• W1969331397 cites W2103867015 @default.
• W1969331397 cites W2128151384 @default.
• W1969331397 cites W2128252882 @default.
• W1969331397 cites W2130728712 @default.
• W1969331397 cites W2134968854 @default.
• W1969331397 cites W2144512576 @default.
• W1969331397 cites W2149912948 @default.
• W1969331397 cites W2157384083 @default.
• W1969331397 cites W2162412306 @default.
• W1969331397 cites W2166625995 @default.
• W1969331397 cites W2168924996 @default.
• W1969331397 cites W2169009248 @default.
• W1969331397 cites W2425956429 @default.
• W1969331397 cites W25687112 @default.
• W1969331397 cites W4212962560 @default.
• W1969331397 doi "https://doi.org/10.1042/bj20020973" @default.
• W1969331397 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1222982" @default.
• W1969331397 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12186630" @default.
• W1969331397 hasPublicationYear "2002" @default.
• W1969331397 type Work @default.
• W1969331397 sameAs 1969331397 @default.
• W1969331397 citedByCount "177" @default.
• W1969331397 countsByYear W19693313972012 @default.
• W1969331397 countsByYear W19693313972013 @default.
• W1969331397 countsByYear W19693313972014 @default.
• W1969331397 countsByYear W19693313972015 @default.
• W1969331397 countsByYear W19693313972016 @default.
• W1969331397 countsByYear W19693313972017 @default.
• W1969331397 countsByYear W19693313972018 @default.
• W1969331397 countsByYear W19693313972019 @default.
• W1969331397 countsByYear W19693313972020 @default.
• W1969331397 countsByYear W19693313972021 @default.
• W1969331397 countsByYear W19693313972022 @default.
• W1969331397 countsByYear W19693313972023 @default.
• W1969331397 crossrefType "journal-article" @default.
• W1969331397 hasAuthorship W1969331397A5020583214 @default.
• W1969331397 hasAuthorship W1969331397A5028009861 @default.
• W1969331397 hasAuthorship W1969331397A5029706709 @default.
• W1969331397 hasAuthorship W1969331397A5035430209 @default.
• W1969331397 hasAuthorship W1969331397A5035453779 @default.
• W1969331397 hasAuthorship W1969331397A5041748356 @default.
• W1969331397 hasAuthorship W1969331397A5054181982 @default.
• W1969331397 hasAuthorship W1969331397A5079162936 @default.
• W1969331397 hasAuthorship W1969331397A5091310822 @default.
• W1969331397 hasBestOaLocation W19693313972 @default.
• W1969331397 hasConcept C11960822 @default.
• W1969331397 hasConcept C134935766 @default.
• W1969331397 hasConcept C150109051 @default.
• W1969331397 hasConcept C153911025 @default.
• W1969331397 hasConcept C2780919815 @default.
• W1969331397 hasConcept C552990157 @default.
• W1969331397 hasConcept C55493867 @default.
• W1969331397 hasConcept C68748289 @default.
• W1969331397 hasConcept C86803240 @default.
• W1969331397 hasConcept C97029542 @default.
• W1969331397 hasConceptScore W1969331397C11960822 @default.

• next