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- W1969337757 abstract "We developed a HPLC method which separates the following nine inositol-containing compounds of biological interest: inositol, inositol 1-monophosphate, inositol 2- or 4-monophosphate, inositol 1,2-cyclic phosphate, inositol 1,4-bisphosphate, inositol 1,4,5-trisphosphate, glycerophosphoinositol, glycerophosphoinositol 4-monophosphate, and glycerophosphoinositol 4,5-bisphosphate. The method shows good resolution and sufficient recovery (70–80%) for each compound. By applying this method to human platelets prelabeled with [3H]inositol and stimulated with thrombin, we found an early increase of inositol 1,4-bisphosphate and inositol 1,4,5-trisphosphate. Accumulation of glycerophosphoinositol, inositol 1-monophosphate, and an inositol monophosphate which cochromatographs with inositol 2- and inositol 4-monophosphate occurs later. The method is simple, and—after removal of salts from the incubation buffer—can be directly applied to the measurement of aqueous soluble [3H]inositol-labeled compounds in biological samples." @default.
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- W1969337757 date "1985-07-01" @default.
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- W1969337757 title "Separation of inositol phosphates and glycerophosphoinositol phosphates by high-performance liquid chromatography" @default.
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- W1969337757 doi "https://doi.org/10.1016/0003-2697(85)90649-9" @default.
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