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• W1969379401 startingPage "1061" @default.
• W1969379401 abstract "The present study investigated the protective effect of methanolic extract from Fuzhuan brick‑tea (FME) on hydrogen peroxide (H2O2)‑induced oxidative stress in the human intestinal epithelial adenocarcinoma cell line Caco‑2. Caco‑2 cells were pretreated with different concentrations (50, 100 and 200 µg/ml) of FME for 2 h and then exposed to H2O2 (1 mM) for 6 h. FME did not exhibit a significant cytotoxic effect and increased the cell viability following H2O2 treatment by decreasing lipid peroxidation in Caco‑2 cells. To investigate the protective effect of FME on H2O2‑induced oxidative stress in Caco‑2 cells, the levels of intracellular glutathione (GSH) and the activity of the endogenous antioxidant enzymes catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH‑px) and glutathione S‑transferase (GST), were determined. FME significantly increased the level of GSH and the activity of antioxidant enzymes. The results from the present study demonstrated that FME has a protective effect on H2O2‑induced oxidative damage in Caco‑2 cells through the inhibition of lipid peroxidation and the increase in the activity of antioxidant enzymes. In addition, FME reduced the H2O2‑induced expression of interleukin‑8 at both the mRNA and protein levels in Caco‑2 cells." @default.
• W1969379401 created "2016-06-24" @default.
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• W1969379401 date "2014-01-07" @default.
• W1969379401 modified "2023-09-29" @default.
• W1969379401 title "Effects of methanolic extract form Fuzhuan brick-tea on hydrogen peroxide-induced oxidative stress in human intestinal epithelial adenocarcinoma Caco-2 cells" @default.
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• W1969379401 doi "https://doi.org/10.3892/mmr.2014.1884" @default.
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