Matches in SemOpenAlex for { <https://semopenalex.org/work/W1969406015> ?p ?o ?g. }
- W1969406015 abstract "In this study we have modified the BioPhotonics workstation (BWS), which allows for using long working distance objective for optical trapping, to include traditional epi-fluorescence microscopy, using the trapping objectives. We have also added temperature regulation of sample stage, allowing for fast temperature variations while trapping. Using this modified BWS setup, we investigated the internal pH (pHi) response and membrane integrity of an optically trapped Saccharomyces cerevisiae cell at 5 mW subject to increasing temperatures. The pHi of the cell is obtained from the emission of 5-(and-6)-carboxyfluorescein diacetate, succinimidyl ester, at 435 and 485 nm wavelengths, while the permeability is indicated by the fluorescence of propidium iodide. We present images mapping the pHi and permeability of the cell at different temperatures and with enough spatial resolution to localize these attributes within the cell. The combined capability of optical trapping, fluorescence microscopy and temperature regulation offers a versatile tool for biological research." @default.
- W1969406015 created "2016-06-24" @default.
- W1969406015 creator A5004601824 @default.
- W1969406015 creator A5013976491 @default.
- W1969406015 creator A5015510278 @default.
- W1969406015 creator A5064838814 @default.
- W1969406015 creator A5086546710 @default.
- W1969406015 date "2011-08-01" @default.
- W1969406015 modified "2023-10-15" @default.
- W1969406015 title "BioPhotonics workstation: A versatile setup for simultaneous optical manipulation, heat stress, and intracellular pH measurements of a live yeast cell" @default.
- W1969406015 cites W1491373961 @default.
- W1969406015 cites W1595593492 @default.
- W1969406015 cites W1601936385 @default.
- W1969406015 cites W1975247969 @default.
- W1969406015 cites W1975545745 @default.
- W1969406015 cites W1979340466 @default.
- W1969406015 cites W2050470027 @default.
- W1969406015 cites W2060519194 @default.
- W1969406015 cites W2063596325 @default.
- W1969406015 cites W2065190374 @default.
- W1969406015 cites W2079594619 @default.
- W1969406015 cites W2084203507 @default.
- W1969406015 cites W2095453725 @default.
- W1969406015 cites W2098358122 @default.
- W1969406015 cites W2110756957 @default.
- W1969406015 cites W2121023572 @default.
- W1969406015 cites W2156814492 @default.
- W1969406015 cites W2158060338 @default.
- W1969406015 cites W2166009034 @default.
- W1969406015 cites W4250818325 @default.
- W1969406015 doi "https://doi.org/10.1063/1.3625274" @default.
- W1969406015 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21895251" @default.
- W1969406015 hasPublicationYear "2011" @default.
- W1969406015 type Work @default.
- W1969406015 sameAs 1969406015 @default.
- W1969406015 citedByCount "6" @default.
- W1969406015 countsByYear W19694060152014 @default.
- W1969406015 countsByYear W19694060152015 @default.
- W1969406015 countsByYear W19694060152016 @default.
- W1969406015 countsByYear W19694060152017 @default.
- W1969406015 countsByYear W19694060152018 @default.
- W1969406015 crossrefType "journal-article" @default.
- W1969406015 hasAuthorship W1969406015A5004601824 @default.
- W1969406015 hasAuthorship W1969406015A5013976491 @default.
- W1969406015 hasAuthorship W1969406015A5015510278 @default.
- W1969406015 hasAuthorship W1969406015A5064838814 @default.
- W1969406015 hasAuthorship W1969406015A5086546710 @default.
- W1969406015 hasBestOaLocation W19694060152 @default.
- W1969406015 hasConcept C113196181 @default.
- W1969406015 hasConcept C120665830 @default.
- W1969406015 hasConcept C121332964 @default.
- W1969406015 hasConcept C12554922 @default.
- W1969406015 hasConcept C147080431 @default.
- W1969406015 hasConcept C1491633281 @default.
- W1969406015 hasConcept C15526883 @default.
- W1969406015 hasConcept C169274487 @default.
- W1969406015 hasConcept C185592680 @default.
- W1969406015 hasConcept C18903297 @default.
- W1969406015 hasConcept C190283241 @default.
- W1969406015 hasConcept C192562407 @default.
- W1969406015 hasConcept C20788544 @default.
- W1969406015 hasConcept C2775934118 @default.
- W1969406015 hasConcept C2777924906 @default.
- W1969406015 hasConcept C2779222958 @default.
- W1969406015 hasConcept C31573885 @default.
- W1969406015 hasConcept C43617362 @default.
- W1969406015 hasConcept C46465826 @default.
- W1969406015 hasConcept C49040817 @default.
- W1969406015 hasConcept C55493867 @default.
- W1969406015 hasConcept C86803240 @default.
- W1969406015 hasConcept C91881484 @default.
- W1969406015 hasConceptScore W1969406015C113196181 @default.
- W1969406015 hasConceptScore W1969406015C120665830 @default.
- W1969406015 hasConceptScore W1969406015C121332964 @default.
- W1969406015 hasConceptScore W1969406015C12554922 @default.
- W1969406015 hasConceptScore W1969406015C147080431 @default.
- W1969406015 hasConceptScore W1969406015C1491633281 @default.
- W1969406015 hasConceptScore W1969406015C15526883 @default.
- W1969406015 hasConceptScore W1969406015C169274487 @default.
- W1969406015 hasConceptScore W1969406015C185592680 @default.
- W1969406015 hasConceptScore W1969406015C18903297 @default.
- W1969406015 hasConceptScore W1969406015C190283241 @default.
- W1969406015 hasConceptScore W1969406015C192562407 @default.
- W1969406015 hasConceptScore W1969406015C20788544 @default.
- W1969406015 hasConceptScore W1969406015C2775934118 @default.
- W1969406015 hasConceptScore W1969406015C2777924906 @default.
- W1969406015 hasConceptScore W1969406015C2779222958 @default.
- W1969406015 hasConceptScore W1969406015C31573885 @default.
- W1969406015 hasConceptScore W1969406015C43617362 @default.
- W1969406015 hasConceptScore W1969406015C46465826 @default.
- W1969406015 hasConceptScore W1969406015C49040817 @default.
- W1969406015 hasConceptScore W1969406015C55493867 @default.
- W1969406015 hasConceptScore W1969406015C86803240 @default.
- W1969406015 hasConceptScore W1969406015C91881484 @default.
- W1969406015 hasIssue "8" @default.
- W1969406015 hasLocation W19694060151 @default.
- W1969406015 hasLocation W19694060152 @default.
- W1969406015 hasLocation W19694060153 @default.
- W1969406015 hasOpenAccess W1969406015 @default.
- W1969406015 hasPrimaryLocation W19694060151 @default.