Matches in SemOpenAlex for { <https://semopenalex.org/work/W1969413610> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W1969413610 endingPage "735" @default.
- W1969413610 startingPage "731" @default.
- W1969413610 abstract "Porphobilinogen deaminase mutants that cause acute intermittent porphyria have been investigated as recombinant proteins expressed in Escherichia coli, yielding important insight into the mechanism of dipyrromethane cofactor assembly and tetrapyrrole chain polymerization. A mutation that affects a key catalytic residue, D99G, results in an inactive holo -protein that exists as a complex with two substrate molecules covalently bound to the dipyrromethane cofactor arising from the reaction between the apo -protein and pre-uroporphyrinogen. The R149Q mutant is also devoid of catalytic activity but the mutant protein is unable to assemble the dipyrromethane cofactor from pre-uroporphyrinogen and persists as an unstable, heat-labile apo -protein. The mutant, R173Q, has very low activity and, like R149Q, also exhibits largely as an apo -protein. The inability to reconstitute either R149Q or R173Q with exogenous pre-uroporphyrinogen confirms the importance of these two arginine residues for dipyrromethane cofactor assembly. In contrast, the mutant R167Q exists as a holo -enzyme but the catalytic cycle is severely compromised, leading to the accumulation of stable enzyme-substrate intermediates from the catalytic cycle." @default.
- W1969413610 created "2016-06-24" @default.
- W1969413610 creator A5003767549 @default.
- W1969413610 creator A5052698759 @default.
- W1969413610 creator A5058690748 @default.
- W1969413610 creator A5074198017 @default.
- W1969413610 creator A5089347931 @default.
- W1969413610 date "2003-06-01" @default.
- W1969413610 modified "2023-09-27" @default.
- W1969413610 title "Human porphobilinogen deaminase mutations in the investigation of the mechanism of dipyrromethane cofactor assembly and tetrapyrrole formation" @default.
- W1969413610 cites W2076389077 @default.
- W1969413610 doi "https://doi.org/10.1042/bst0310731" @default.
- W1969413610 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12773194" @default.
- W1969413610 hasPublicationYear "2003" @default.
- W1969413610 type Work @default.
- W1969413610 sameAs 1969413610 @default.
- W1969413610 citedByCount "29" @default.
- W1969413610 countsByYear W19694136102012 @default.
- W1969413610 countsByYear W19694136102013 @default.
- W1969413610 countsByYear W19694136102014 @default.
- W1969413610 countsByYear W19694136102016 @default.
- W1969413610 countsByYear W19694136102018 @default.
- W1969413610 countsByYear W19694136102021 @default.
- W1969413610 countsByYear W19694136102022 @default.
- W1969413610 crossrefType "journal-article" @default.
- W1969413610 hasAuthorship W1969413610A5003767549 @default.
- W1969413610 hasAuthorship W1969413610A5052698759 @default.
- W1969413610 hasAuthorship W1969413610A5058690748 @default.
- W1969413610 hasAuthorship W1969413610A5074198017 @default.
- W1969413610 hasAuthorship W1969413610A5089347931 @default.
- W1969413610 hasConcept C104317684 @default.
- W1969413610 hasConcept C143065580 @default.
- W1969413610 hasConcept C181199279 @default.
- W1969413610 hasConcept C185592680 @default.
- W1969413610 hasConcept C197957613 @default.
- W1969413610 hasConcept C2776217839 @default.
- W1969413610 hasConcept C2776682811 @default.
- W1969413610 hasConcept C2777710969 @default.
- W1969413610 hasConcept C2778503633 @default.
- W1969413610 hasConcept C55493867 @default.
- W1969413610 hasConcept C63338738 @default.
- W1969413610 hasConceptScore W1969413610C104317684 @default.
- W1969413610 hasConceptScore W1969413610C143065580 @default.
- W1969413610 hasConceptScore W1969413610C181199279 @default.
- W1969413610 hasConceptScore W1969413610C185592680 @default.
- W1969413610 hasConceptScore W1969413610C197957613 @default.
- W1969413610 hasConceptScore W1969413610C2776217839 @default.
- W1969413610 hasConceptScore W1969413610C2776682811 @default.
- W1969413610 hasConceptScore W1969413610C2777710969 @default.
- W1969413610 hasConceptScore W1969413610C2778503633 @default.
- W1969413610 hasConceptScore W1969413610C55493867 @default.
- W1969413610 hasConceptScore W1969413610C63338738 @default.
- W1969413610 hasIssue "3" @default.
- W1969413610 hasLocation W19694136101 @default.
- W1969413610 hasLocation W19694136102 @default.
- W1969413610 hasOpenAccess W1969413610 @default.
- W1969413610 hasPrimaryLocation W19694136101 @default.
- W1969413610 hasRelatedWork W1645792073 @default.
- W1969413610 hasRelatedWork W1967528101 @default.
- W1969413610 hasRelatedWork W1969413610 @default.
- W1969413610 hasRelatedWork W1984123806 @default.
- W1969413610 hasRelatedWork W1993084091 @default.
- W1969413610 hasRelatedWork W2019089536 @default.
- W1969413610 hasRelatedWork W2155013091 @default.
- W1969413610 hasRelatedWork W258403680 @default.
- W1969413610 hasRelatedWork W2786705521 @default.
- W1969413610 hasRelatedWork W4362608702 @default.
- W1969413610 hasVolume "31" @default.
- W1969413610 isParatext "false" @default.
- W1969413610 isRetracted "false" @default.
- W1969413610 magId "1969413610" @default.
- W1969413610 workType "article" @default.