Matches in SemOpenAlex for { <https://semopenalex.org/work/W1969428610> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W1969428610 endingPage "363" @default.
- W1969428610 startingPage "351" @default.
- W1969428610 abstract "γ-Aminobutyric acid (GABA) receptor was solubilized from synaptic membrane of the rat brain by various detergents. Nonidet P-40, a non-ionic detergent, was found to be an effective solubilizing agent, since it caused no interference on the receptor binding assay, yielded a [3H]muscimol binding protein with a high specific activity and no aggregation, and preserved good stability of the solubilized fraction. Ammonium sulfate precipitation of the solubilized supernatant significantly increased the binding of [3H]muscimol to GABA receptor, possibly by removing heat-stable and small molecular inhibitory substances. The specific [3H]muscimol binding to the soluble fraction obtained by Nonidet P-40 treatment and subsequent ammonium sulfate precipitation, was saturable withKD 13 and 64 nM, and Bmax 3.4 and 1.8 pmol/mg protein, respectively. The enhancement of the [3H]muscimol binding by diazepam as found in synaptic membrane was also detected in the soluble fraction. Molecular weight of the [3H]muscimol binding site was determined by gel filtration on Sephadex G-200 and was calculated to be 270,000 daltons. This value was identical with that of the [3H]flunitrazepam binding site which appeared in the same solubilized fraction. These results indicate that the properties of solubilized GABA receptor are identical to those of membrane-bound GABA receptor. Furthermore, the present results suggest that both GABA and benzodiazepine receptors may reside on the same macromolecule in synaptic membrane." @default.
- W1969428610 created "2016-06-24" @default.
- W1969428610 creator A5027191467 @default.
- W1969428610 creator A5074545551 @default.
- W1969428610 date "1982-03-01" @default.
- W1969428610 modified "2023-10-16" @default.
- W1969428610 title "Some properties of solubilized GABA receptor" @default.
- W1969428610 cites W1775749144 @default.
- W1969428610 cites W1975875808 @default.
- W1969428610 cites W1975946135 @default.
- W1969428610 cites W1977458557 @default.
- W1969428610 cites W1978475206 @default.
- W1969428610 cites W1978782104 @default.
- W1969428610 cites W1979744268 @default.
- W1969428610 cites W1980350256 @default.
- W1969428610 cites W1985868885 @default.
- W1969428610 cites W1993405721 @default.
- W1969428610 cites W2036468370 @default.
- W1969428610 cites W2040855890 @default.
- W1969428610 cites W2053939453 @default.
- W1969428610 cites W2060226757 @default.
- W1969428610 cites W2066483240 @default.
- W1969428610 cites W2072957692 @default.
- W1969428610 cites W2087773637 @default.
- W1969428610 cites W2090103911 @default.
- W1969428610 cites W2090447712 @default.
- W1969428610 cites W2123263864 @default.
- W1969428610 cites W2124717311 @default.
- W1969428610 doi "https://doi.org/10.1016/0006-8993(82)90720-x" @default.
- W1969428610 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6279240" @default.
- W1969428610 hasPublicationYear "1982" @default.
- W1969428610 type Work @default.
- W1969428610 sameAs 1969428610 @default.
- W1969428610 citedByCount "44" @default.
- W1969428610 crossrefType "journal-article" @default.
- W1969428610 hasAuthorship W1969428610A5027191467 @default.
- W1969428610 hasAuthorship W1969428610A5074545551 @default.
- W1969428610 hasConcept C12554922 @default.
- W1969428610 hasConcept C163994747 @default.
- W1969428610 hasConcept C168258287 @default.
- W1969428610 hasConcept C170493617 @default.
- W1969428610 hasConcept C178790620 @default.
- W1969428610 hasConcept C181199279 @default.
- W1969428610 hasConcept C185592680 @default.
- W1969428610 hasConcept C193363816 @default.
- W1969428610 hasConcept C2776428949 @default.
- W1969428610 hasConcept C2779188335 @default.
- W1969428610 hasConcept C2779870022 @default.
- W1969428610 hasConcept C2781442855 @default.
- W1969428610 hasConcept C41625074 @default.
- W1969428610 hasConcept C43617362 @default.
- W1969428610 hasConcept C4863142 @default.
- W1969428610 hasConcept C55493867 @default.
- W1969428610 hasConcept C86803240 @default.
- W1969428610 hasConceptScore W1969428610C12554922 @default.
- W1969428610 hasConceptScore W1969428610C163994747 @default.
- W1969428610 hasConceptScore W1969428610C168258287 @default.
- W1969428610 hasConceptScore W1969428610C170493617 @default.
- W1969428610 hasConceptScore W1969428610C178790620 @default.
- W1969428610 hasConceptScore W1969428610C181199279 @default.
- W1969428610 hasConceptScore W1969428610C185592680 @default.
- W1969428610 hasConceptScore W1969428610C193363816 @default.
- W1969428610 hasConceptScore W1969428610C2776428949 @default.
- W1969428610 hasConceptScore W1969428610C2779188335 @default.
- W1969428610 hasConceptScore W1969428610C2779870022 @default.
- W1969428610 hasConceptScore W1969428610C2781442855 @default.
- W1969428610 hasConceptScore W1969428610C41625074 @default.
- W1969428610 hasConceptScore W1969428610C43617362 @default.
- W1969428610 hasConceptScore W1969428610C4863142 @default.
- W1969428610 hasConceptScore W1969428610C55493867 @default.
- W1969428610 hasConceptScore W1969428610C86803240 @default.
- W1969428610 hasIssue "2" @default.
- W1969428610 hasLocation W19694286101 @default.
- W1969428610 hasLocation W19694286102 @default.
- W1969428610 hasOpenAccess W1969428610 @default.
- W1969428610 hasPrimaryLocation W19694286101 @default.
- W1969428610 hasRelatedWork W1973189354 @default.
- W1969428610 hasRelatedWork W1994009629 @default.
- W1969428610 hasRelatedWork W2059766593 @default.
- W1969428610 hasRelatedWork W2060257694 @default.
- W1969428610 hasRelatedWork W2083989983 @default.
- W1969428610 hasRelatedWork W2084543862 @default.
- W1969428610 hasRelatedWork W2146860076 @default.
- W1969428610 hasRelatedWork W2170238590 @default.
- W1969428610 hasRelatedWork W2271317955 @default.
- W1969428610 hasRelatedWork W2339266019 @default.
- W1969428610 hasVolume "236" @default.
- W1969428610 isParatext "false" @default.
- W1969428610 isRetracted "false" @default.
- W1969428610 magId "1969428610" @default.
- W1969428610 workType "article" @default.