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• W1969432884 abstract "Fluorescence spectroscopy, a very sensitive index for measuring the biophysical properties of living cell systems, was used to examine the structural order of intact, resting, gel-filtered platelets from hyperlipidemic subjects (n = 48, 25–70 years) and normolipemic subjects (n = 34, 19–68 years). Fluorescence anisotropy (r[s]), which is inversely related to membrane fluidity, was estimated using 3 different fluorescent dyes, DPH, TMA-DPH, and 6-AS, known to label different regions of biological membranes. Increased membrane fluidity was observed in type IIB (n = 24, 36–62 yrs; r[s] = 0.0692 ± 0.09) and type IV (n = 10, 33–57 yrs; r[s] = 0.058 ± 0.006) hyperlipidemics in comparison to type IIA (n = 14, 25–70 yrs; r[s] = 0.086 ± 0.019) and control subjects (n = 24, 28–68 yrs; r[s] = 0.079 ± 0.012). The temperature dependency of r[s]-DPH values was significantly different (P < 0.01) in platelets from type IIB and type IV patients compared to type IIA and control subjects of similar age. A significant positive correlation (P < 0.005) between membrane fluidity and age was found only in healthy control subjects (n = 34, 19–68 yrs). Despite significant (P < 0.01) differences in plasma lipid concentrations in hyperlipidemic patients and controls, significant ex vivo relations between membrane fluidity and lipoprotein concentrations, free fatty acid distribution, and increased age were found only in healthy control subjects. Plasma levels of thromboxane as well as serum selenium concentrations did not significantly differ between hypercholesterolemic, hypertriglyceridemic, and control subjects." @default.
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• W1969432884 date "1991-04-01" @default.
• W1969432884 modified "2023-10-09" @default.
• W1969432884 title "Platelet membrane fluidity in type IIA, type IIB and type IV hyperlipoproteinemia" @default.
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• W1969432884 doi "https://doi.org/10.1016/0021-9150(91)90018-x" @default.
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