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- W1969489135 abstract "Dientamoeba fragilis is a trichomonad parasite that causes human gastrointestinal disease. Currently microscopy is considered to be the gold standard for diagnosis of D. fragilis infection. However, this method is time-consuming and relatively insensitive. A PCR assay based on the small-subunit ribosomal RNA gene of D. fragilis for the specific detection of D. fragilis DNA in fresh unpreserved stool samples was developed. The D. fragilis PCR was positive in 29/31 samples with positive microscopy and did not cross-react with other protozoan parasites. The PCR protocol showed a specificity of 100% and a sensitivity of 93.5% and the entire procedure can be performed in one day." @default.
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- W1969489135 date "2005-01-01" @default.
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- W1969489135 title "Detection of Dientamoeba fragilis in fresh stool specimens using PCR" @default.
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- W1969489135 doi "https://doi.org/10.1016/j.ijpara.2004.09.003" @default.
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