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- W1969532336 abstract "Genetic polymorphisms in the alcohol dehydrogenase genes, ADH2 and ADH3, have been shown to affect individual susceptibility to diseases such as alcoholism and oesophageal cancer. Although several PCR-based methods for genotyping these enzymes have been previously developed, the two-buffer polyacrylamide gel electrophoresis system has the ability to rapidly resolve all classes of point mutations within 2-3 hours instead of the conventional overnight separation. The success of this technique is partly attributable to a discontinuous two-phase buffer system and horizontal flatbed gel electrophoresis rather than conventional vertical gels. Using a modification of this system, we were able to detect all of the known polymorphisms within ADH2 exons 3 and 9 and ADH3 exon 8, as well as a rare variant within ADH2 exon 9. This method is rapid, cost-effective, and is ideal for large scale screening programmes." @default.
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- W1969532336 date "2003-01-24" @default.
- W1969532336 modified "2023-09-23" @default.
- W1969532336 title "Genotyping of Alcohol Dehydrogenase Type 2 and 3 Using a Two-Buffer Polyacrylamide Gel Electrophoresis System" @default.
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- W1969532336 doi "https://doi.org/10.1515/cclm.2003.047" @default.
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