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- W1969551659 abstract "Potent nitric oxide (NO) signals are described for many forms of cell-cell communication. Although NO plays a significant role in skeletal muscle metabolism and contractility and in precursor activation during muscle formation and stretching, there is no direct evidence of stretch-induced NO release from muscle. Differentiated muscle cell cultures from normal and dystrophic mdx mice were preloaded with the NO-specific dye DAF-2 (diaminofluorescein-2) before stretching. NO release was detected by video-microscopy. NO was released rapidly from wild-type (WT) cells after stretch and intensity declined rapidly to a plateau. Mdx cells showed much less NO release. Direct observations of the time-course of stretch-induced NO release in WT cells is congruent with the hypothesis of NO-mediated stretch activation of satellite cells in normal skeletal muscle. Distinct differences in the time-course between normal and dystrophic cells indicate visualization methods for NO release will be a sensitive measure of NOS-1 restoration following diverse treatment approaches to muscular dystrophy." @default.
- W1969551659 created "2016-06-24" @default.
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- W1969551659 date "2009-03-01" @default.
- W1969551659 modified "2023-10-16" @default.
- W1969551659 title "The dynamics of the nitric oxide release-transient from stretched muscle cells" @default.
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- W1969551659 doi "https://doi.org/10.1016/j.biocel.2008.07.005" @default.
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