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- W1969597332 endingPage "634" @default.
- W1969597332 startingPage "621" @default.
- W1969597332 abstract "Chronic hypoxia is a common clinical event that induces adaptive responses and can result in behavioral deterioration. The reduction of metabolic rate during hypoxia may limit overall protein phosphorylation owing to the lack of high energy phosphate. However, the hypoxia-induced regulation of phosphoproteins is poorly understood. Here, we characterized the CNS phosphoproteome of Lymnaea stagnalis, a freshwater snail that has been used as a model to study chronic hypoxia-induced neural depression. After hypoxia treatment for 4 days, the motor behavior of the snail was suppressed. Electrophysiological measurements from Pedal A (PeA) interneurons showed that hypoxia increased the frequency of spontaneous postsynaptic excitatory potentials (sEPSPs), but reduced the firing frequency, the amplitude, and the half-width duration (APD50) of spontaneous action potentials. Imaging with a fluorescent phosphate label, Pro-Q Diamond, revealed that the neuronal phosphoprotein level was reduced after the hypoxia treatment. The hypoxia-induced changes in the phosphoproteome of the central ganglia were quantified using one-dimensional gel-electrophoresis by comparing the fluorescence intensity ratio of phospholabeled phosphoproteins versus total proteins between the hypoxia and control groups. We analyzed 16 protein bands: eight showed decreased phosphorylation levels after hypoxia treatment, and eight did not change. Using mass spectrometry analysis and protein database matching we found three phosphoproteins that may be associated with chronic hypoxia-induced neuronal adaptive response of the snail. This is the first proteomic screening for neural phosphoproteins in chronic hypoxia." @default.
- W1969597332 created "2016-06-24" @default.
- W1969597332 creator A5006027867 @default.
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- W1969597332 creator A5071323598 @default.
- W1969597332 creator A5082491359 @default.
- W1969597332 creator A5089102437 @default.
- W1969597332 date "2009-06-01" @default.
- W1969597332 modified "2023-10-18" @default.
- W1969597332 title "Neural phosphoproteomics of a chronic hypoxia model—Lymnaea stagnalis" @default.
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- W1969597332 doi "https://doi.org/10.1016/j.neuroscience.2009.03.043" @default.
- W1969597332 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19324076" @default.