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- W1969637576 abstract "DNA-proteins interactions can be studied in great detail when precise and dynamic control of the mechanical state of a single DNA molecule can be achieved together with direct visualization of proteins interacting with the DNA. Our custom-built experimental setup combines optical trapping and wide-field epifluorescence microscopy. In this setup we can visualize single DNA-bound proteins (see figure) with an accuracy of tens of nanometers while at the same time we can control the tension applied on the DNA at a sub-PicoNewton level. Here we report the limit of localization accuracy in combined DNA-trapping/fluorescence experiments by fitting a point-spread function to the fluorescence image of single DNA-bound proteins. We investigate the impact of DNA dynamics on the maximum attainable accuracy for the localization of these DNA-bound fluorescent proteins (in this case a Ca2+ inactivated restriction enzyme EcoRV). In particular, we study the effect of tension on the DNA and identify the force regime in which single-proteins can be localized with super-resolution." @default.
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- W1969637576 date "2009-02-01" @default.
- W1969637576 modified "2023-10-14" @default.
- W1969637576 title "Visualizing Single-proteins On A Single DNA Molecule With Super-resolution" @default.
- W1969637576 doi "https://doi.org/10.1016/j.bpj.2008.12.2881" @default.
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