FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1969724874> ?p ?o ?g. }

• W1969724874 endingPage "2692" @default.
• W1969724874 startingPage "2685" @default.
• W1969724874 abstract "To examine the importance of reduced intracellular glutathione (GSH) in the modulation of dysmorphogenesis and to gain insight into the electrophilic character of the embryotoxic intermediates generated in the rat embryo from N-acetoxy-2-acetylaminofluorene (AAAF) and acetaminophen (APAP) in cultured embryos, the effects of GSH depletion on the embryotoxicity, dysmorphogenesis and covalent binding of these agents were examined. Both AAAF (90 microM) and APAP (500 microM) produced concentration-dependent, statistically significant (P less than or equal to 0.05) decreases in embryonic length as well as embryonic and visceral yolk sac protein content when rat embryos were exposed in vitro between days 10 and 11 of gestation. The predominant malformations observed upon exposure to AAAF and APAP were prosencephalic hypoplasia and abnormal neurulation respectively. Exposure of conceptuses to [3H]APAP followed by separation and fractionation of the cellular RNA, DNA and protein via density gradient centrifugation resulted in detectable binding in fractions that contained protein, but not DNA or RNA. This suggested that the rat conceptus is capable of bioactivating APAP to a soft electrophile that selectively arylates protein. In contrast, conceptuses exposed to [3H]AAAF exhibited detectable binding to RNA, DNA and protein, indicative of conversion to both hard and soft electrophiles. Depletion of GSH was accomplished by pretreating conceptuses with 500 microM L-buthionine-S,R-sulfoximine (BSO) from the start of the culture period (day 9.5) until the morning of day 10. When conceptuses were depleted previously of GSH by BSO, exposure to APAP resulted in significant potentiation (relative to APAP alone) of the observed embryotoxicity. These conceptuses displayed further decreases in both embryonic size and protein content of the embryo and yolk sac, as well as increased incidence of abnormally open anterior neuropores and increased binding (3-fold) of [3H]APAP to protein. In contrast, pretreatment with BSO did not potentiate the AAAF-elicited decreases in embryonic size or protein content, nor the severity of prosencephalic hypoplasia, although a slight increase in binding of [3H]AAAF to DNA was observed. Taken together, these data are consistent with the concept that abnormal neurulation elicited by APAP results from the generation of one or more soft electrophilic species, whereas elicitation of prosencephalic hypoplasia by AAAF appears to be a consequence of conversion to a relatively hard electrophile(s)." @default.
• W1969724874 created "2016-06-24" @default.
• W1969724874 creator A5023884929 @default.
• W1969724874 creator A5024760651 @default.
• W1969724874 creator A5024804968 @default.
• W1969724874 date "1989-08-01" @default.
• W1969724874 modified "2023-10-18" @default.
• W1969724874 title "Influence of electrophilic character and glutathione depletion on chemical dysmorphogenesis in cultured rat embryos" @default.
• W1969724874 cites W1969352130 @default.
• W1969724874 cites W1973475159 @default.
• W1969724874 cites W1977779461 @default.
• W1969724874 cites W1978584770 @default.
• W1969724874 cites W1982059177 @default.
• W1969724874 cites W1984099932 @default.
• W1969724874 cites W1986266245 @default.
• W1969724874 cites W1992308157 @default.
• W1969724874 cites W199626244 @default.
• W1969724874 cites W2009357638 @default.
• W1969724874 cites W2017246842 @default.
• W1969724874 cites W2027206386 @default.
• W1969724874 cites W2027320080 @default.
• W1969724874 cites W2032736194 @default.
• W1969724874 cites W2034291477 @default.
• W1969724874 cites W2036973195 @default.
• W1969724874 cites W2046586101 @default.
• W1969724874 cites W2053415398 @default.
• W1969724874 cites W2057090998 @default.
• W1969724874 cites W2062178075 @default.
• W1969724874 cites W2063148356 @default.
• W1969724874 cites W2063568463 @default.
• W1969724874 cites W2072922607 @default.
• W1969724874 cites W2074849399 @default.
• W1969724874 cites W2077942454 @default.
• W1969724874 cites W2093793222 @default.
• W1969724874 cites W2094773643 @default.
• W1969724874 cites W2115161371 @default.
• W1969724874 cites W2158577892 @default.
• W1969724874 cites W2481752616 @default.
• W1969724874 cites W3004880170 @default.
• W1969724874 cites W4251825801 @default.
• W1969724874 cites W4293247451 @default.
• W1969724874 doi "https://doi.org/10.1016/0006-2952(89)90555-8" @default.
• W1969724874 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2764988" @default.
• W1969724874 hasPublicationYear "1989" @default.
• W1969724874 type Work @default.
• W1969724874 sameAs 1969724874 @default.
• W1969724874 citedByCount "21" @default.
• W1969724874 countsByYear W19697248742017 @default.
• W1969724874 countsByYear W19697248742022 @default.
• W1969724874 crossrefType "journal-article" @default.
• W1969724874 hasAuthorship W1969724874A5023884929 @default.
• W1969724874 hasAuthorship W1969724874A5024760651 @default.
• W1969724874 hasAuthorship W1969724874A5024804968 @default.
• W1969724874 hasConcept C126322002 @default.
• W1969724874 hasConcept C134018914 @default.
• W1969724874 hasConcept C153911025 @default.
• W1969724874 hasConcept C16685009 @default.
• W1969724874 hasConcept C181199279 @default.
• W1969724874 hasConcept C196843134 @default.
• W1969724874 hasConcept C2777043721 @default.
• W1969724874 hasConcept C2777476445 @default.
• W1969724874 hasConcept C2778128677 @default.
• W1969724874 hasConcept C2779234561 @default.
• W1969724874 hasConcept C2779335785 @default.
• W1969724874 hasConcept C32253518 @default.
• W1969724874 hasConcept C3675279 @default.
• W1969724874 hasConcept C46973012 @default.
• W1969724874 hasConcept C538909803 @default.
• W1969724874 hasConcept C54355233 @default.
• W1969724874 hasConcept C55493867 @default.
• W1969724874 hasConcept C71924100 @default.
• W1969724874 hasConcept C86803240 @default.
• W1969724874 hasConcept C87073359 @default.
• W1969724874 hasConcept C95444343 @default.
• W1969724874 hasConceptScore W1969724874C126322002 @default.
• W1969724874 hasConceptScore W1969724874C134018914 @default.
• W1969724874 hasConceptScore W1969724874C153911025 @default.
• W1969724874 hasConceptScore W1969724874C16685009 @default.
• W1969724874 hasConceptScore W1969724874C181199279 @default.
• W1969724874 hasConceptScore W1969724874C196843134 @default.
• W1969724874 hasConceptScore W1969724874C2777043721 @default.
• W1969724874 hasConceptScore W1969724874C2777476445 @default.
• W1969724874 hasConceptScore W1969724874C2778128677 @default.
• W1969724874 hasConceptScore W1969724874C2779234561 @default.
• W1969724874 hasConceptScore W1969724874C2779335785 @default.
• W1969724874 hasConceptScore W1969724874C32253518 @default.
• W1969724874 hasConceptScore W1969724874C3675279 @default.
• W1969724874 hasConceptScore W1969724874C46973012 @default.
• W1969724874 hasConceptScore W1969724874C538909803 @default.
• W1969724874 hasConceptScore W1969724874C54355233 @default.
• W1969724874 hasConceptScore W1969724874C55493867 @default.
• W1969724874 hasConceptScore W1969724874C71924100 @default.
• W1969724874 hasConceptScore W1969724874C86803240 @default.
• W1969724874 hasConceptScore W1969724874C87073359 @default.
• W1969724874 hasConceptScore W1969724874C95444343 @default.
• W1969724874 hasIssue "16" @default.
• W1969724874 hasLocation W19697248741 @default.
• W1969724874 hasLocation W19697248742 @default.

• next