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- W1969736675 abstract "Assay of proteolytic enzymes by the fluorescence polarization method is described in which fluorescein isothiocyanate-conjugated proteins are used as substrates. Hydrolysis of the natural substrates is accompanied by a decrease in the fluorescence polarization values reflecting time and also by an enzyme concentration-dependent decrease in the overall molecular weight of the substrates. Trypsin activity as low as 0.05 μg/ml at 20°C is detected at a fluorochrome concentration of 10 to 20 pmol/ml. In this method, no separation procedure or further reaction for quantitation is necessary. Thus, using a computerized instrument, this method is extremely sensitive, simple, and rapid." @default.
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- W1969736675 date "1979-01-01" @default.
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- W1969736675 title "Assay of proteolytic enzymes by the fluorescence polarization technique" @default.
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- W1969736675 doi "https://doi.org/10.1016/0003-2697(79)90649-3" @default.
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