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• W1969774753 abstract "Temperature-dependent fluorescence for intact cells of cyanobacterium Spirulina platensis was detected to search for the connection of the phycobilisome (PBS) with Photosystem I (PSI) and Photosystem II (PSII). Some interesting results were obtained from the deconvoluted fluorescence components of C-phycocyanin (C-PC), allophycocyanin (APC), PSI and PSII as well as the fluorescence spectra of the intact cells at room temperature (RT=25 °C) and 0 °C. It was observed that, compared to those at RT, both of the fluorescence components for PSI and APC increased, whereas those for PSII and C-PC decreased at 0 °C with excitation at 580 nm, that is, the fluorescence for C-PC is not synchronous with that for APC, and the fluorescence fluctuation for PSI is not synchronous with that for PSII. On the other hand, the decrease in C-PC fluorescence is synchronous with the increase in PSI fluorescence, and the increase in APC fluorescence is synchronous with the decrease in PSII fluorescence. Therefore, it can be readily deduced that PBS should be coupled not only with PSII through the terminal acceptors in the APC core but also with PSI through C-PC in PBS rods at physiological condition, while at 0 °C, a migration of a PBS makes the APC partially detached from PSII but the C-PC more efficiently coupled with PSI. The results provide good evidences for “mobile PBS” model and “parallel connection” model but not for the “spillover” model." @default.
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• W1969774753 date "2003-03-01" @default.
• W1969774753 modified "2023-10-16" @default.
• W1969774753 title "Probing connection of PBS with the photosystems in intact cells of Spirulina platensis by temperature-induced fluorescence fluctuation" @default.
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• W1969774753 doi "https://doi.org/10.1016/s0005-2728(02)00392-4" @default.
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