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- W1969793707 abstract "Recent reports have identified an endogenous peptide ligand for the opioid receptor-like (ORL1) receptor. In the present study, ORL1 peptide-stimulated [35>]GTP gamma S binding was assessed in rat cortical membranes and brain sections to localize ORL1 receptor-activated G-proteins. In membrane assays, with 20 microM GDP, ORL1 peptide stimulated [35S]GTP gamma S binding by approximately two-fold with an ED50 value of 20 nM. ORL1 peptide-stimulated [35S]GTP gamma S binding was unaffected by opioid or other G-protein-coupled receptor antagonists. In brain sections, ORL1 peptide-stimulated [35S]GTP gamma S binding was identified in regions including cortex, amygdala, hypothalamus, thalamus and brain stem. The anatomical distribution of ORL1 peptide-stimulated [35S]GTP gamma S binding suggests its involvement in cognition, emotion and homeostasis." @default.
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- W1969793707 date "1996-02-01" @default.
- W1969793707 modified "2023-09-26" @default.
- W1969793707 title "Identification of opioid receptor-like (ORL1) peptide-stimulated [35S]GTPγS binding in rat brain" @default.
- W1969793707 doi "https://doi.org/10.1097/00001756-199602290-00012" @default.
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