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- W1969804548 abstract "The standard method of immunomagnetic separation for isolating pathogenic bacteria from food and environmental matrices processes 1 ml volumes. Pathogens present at low levels (<0.5 pathogenic bacteria/g) will not be consistently detected by this method. Here a multiple sample flow through immunomagnetic separator has been designed and tested to process large volume samples (50 to 250 ml). Preliminary results show >97% recovery of polydisperse magnetic particles (diameter range 1 to 8 microm) containing 29-33% w/w Fe3O4 content. Between 70 and 130 times more of the pathogenic bacteria Escherichia coli O157 is recovered from PBS compared with the standard 1 ml method. Also, the recovery of E. coli O157 from beef mince homogenates, after a 4 h incubation at 42 degrees C, is between 80 and 180 times higher than the standard 1 ml method." @default.
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- W1969804548 date "2005-06-01" @default.
- W1969804548 modified "2023-09-24" @default.
- W1969804548 title "Multiple sample flow through immunomagnetic separator for concentrating pathogenic bacteria" @default.
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- W1969804548 doi "https://doi.org/10.1088/0031-9155/50/12/016" @default.
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