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Matches in SemOpenAlex for { <https://semopenalex.org/work/W1969825706> ?p ?o ?g. }

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• W1969825706 abstract "A DNA encoding thioredoxin-mature carp ovarian cystatin (trx-cystatin) fusion protein was ligated into a pET-23a(+) expression vector and then transformed into Escherichia coli AD494(DE3) expression host. After induction by isopropyl beta-D-thiogalactopyranoside, a high level of the soluble form of recombinant trx-cystatin was expressed in the cytoplasm of E. coli. The recombinant trx-cystatin could be purified by Ni(2+)-NTA agarose affinity chromatography. The molecular mass (M) of the recombinant trx-cystatin was approximately 28 kDa composed of recombinant thioredoxin (16 kDa) and recombinant mature carp ovarian cystatin (12 kDa). Both recombinant trx-fused and mature carp ovarian cystatins were stable at pH 6-11. No obvious decrease in activity was observed even after 5 min of incubation at 60 degrees C. They exhibited papain-like protease inhibition activity comparable to that of the mature carp ovarian cystatin, which could inhibit papain and mackerel cathepsins L and L-like, but not cathepsin B." @default.
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• W1969825706 date "2001-08-30" @default.
• W1969825706 modified "2023-09-23" @default.
• W1969825706 title "Expression of Soluble Form Carp (<i>Cyprinus carpio</i>) Ovarian Cystatin in <i>Escherichia coli</i> and Its Purification" @default.
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• W1969825706 doi "https://doi.org/10.1021/jf0105135" @default.
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