FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1969863232> ?p ?o ?g. }

• W1969863232 endingPage "1227" @default.
• W1969863232 startingPage "1219" @default.
• W1969863232 abstract "Histone H1 isoforms isolated from asynchronously grown HeLa cells were subjected to enzymatic digestion and analyzed by nano-flow reversed-phase high performance liquid chromatography (RP-HPLC) tandem mass spectrometry (MS/MS) on both quadrupole ion trap and linear quadrupole ion trap-Fourier transform ion cyclotron resonance mass spectrometers. We have observed all five major isoforms of histone H1 (H1.1, H1.2, H1.3, H1.4, and H1.5) as well as a lesser studied H1, isoform H1.X. MS/MS experiments confirmed N-terminal acetylation on all isoforms plus a single internal acetylation site. Immobilized metal affinity chromatography in combination with tandem mass spectrometry was utilized to identify 19 phosphorylation sites on the five major H1 isoforms plus H1.X. Fourteen of these phosphorylation sites were located on peptides containing the cyclin dependent kinase (CDK) consensus motif (S/T)−P−X−Z (where X is any amino acid and Z is a basic amino acid). Five phosphorylation sites were identified in regions that did not fit the consensus CDK motif. One of these phosphorylation sites was found on the serine residue on the H1.4 peptide KARKSAGAAKR. The adjacent lysine residue to the phosphoserine was also shown to be methylated. This finding raises the question of whether the hypothesized “methyl/phos” switch could be extended to linker histones, and not exclusive to core histones. Keywords: linear quadrupole ion trap • Fourier transform mass spectrometer • histone H1 • post-translational modifications • phosphorylation • acetylation • methylation • histone code" @default.
• W1969863232 created "2016-06-24" @default.
• W1969863232 creator A5007956433 @default.
• W1969863232 creator A5011931811 @default.
• W1969863232 creator A5013168741 @default.
• W1969863232 creator A5024411219 @default.
• W1969863232 creator A5033126095 @default.
• W1969863232 creator A5058863432 @default.
• W1969863232 date "2004-10-02" @default.
• W1969863232 modified "2023-09-27" @default.
• W1969863232 title "Characterization of Phosphorylation Sites on Histone H1 Isoforms by Tandem Mass Spectrometry" @default.
• W1969863232 cites W1538501027 @default.
• W1969863232 cites W1543553963 @default.
• W1969863232 cites W1646144336 @default.
• W1969863232 cites W1971249969 @default.
• W1969863232 cites W1971437289 @default.
• W1969863232 cites W1973968422 @default.
• W1969863232 cites W1988294401 @default.
• W1969863232 cites W1990323774 @default.
• W1969863232 cites W1993633154 @default.
• W1969863232 cites W2004704197 @default.
• W1969863232 cites W2012119941 @default.
• W1969863232 cites W2021495920 @default.
• W1969863232 cites W2027788169 @default.
• W1969863232 cites W2028445104 @default.
• W1969863232 cites W2030259380 @default.
• W1969863232 cites W2047837922 @default.
• W1969863232 cites W2049118388 @default.
• W1969863232 cites W2054969325 @default.
• W1969863232 cites W2055397241 @default.
• W1969863232 cites W2067724937 @default.
• W1969863232 cites W2068718457 @default.
• W1969863232 cites W2070030633 @default.
• W1969863232 cites W2070312499 @default.
• W1969863232 cites W2076446466 @default.
• W1969863232 cites W2078943658 @default.
• W1969863232 cites W2085677283 @default.
• W1969863232 cites W2098536022 @default.
• W1969863232 cites W2143658931 @default.
• W1969863232 cites W2144792857 @default.
• W1969863232 cites W2158842827 @default.
• W1969863232 doi "https://doi.org/10.1021/pr0498887" @default.
• W1969863232 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15595731" @default.
• W1969863232 hasPublicationYear "2004" @default.
• W1969863232 type Work @default.
• W1969863232 sameAs 1969863232 @default.
• W1969863232 citedByCount "122" @default.
• W1969863232 countsByYear W19698632322012 @default.
• W1969863232 countsByYear W19698632322013 @default.
• W1969863232 countsByYear W19698632322014 @default.
• W1969863232 countsByYear W19698632322015 @default.
• W1969863232 countsByYear W19698632322016 @default.
• W1969863232 countsByYear W19698632322017 @default.
• W1969863232 countsByYear W19698632322018 @default.
• W1969863232 countsByYear W19698632322020 @default.
• W1969863232 countsByYear W19698632322021 @default.
• W1969863232 countsByYear W19698632322022 @default.
• W1969863232 countsByYear W19698632322023 @default.
• W1969863232 crossrefType "journal-article" @default.
• W1969863232 hasAuthorship W1969863232A5007956433 @default.
• W1969863232 hasAuthorship W1969863232A5011931811 @default.
• W1969863232 hasAuthorship W1969863232A5013168741 @default.
• W1969863232 hasAuthorship W1969863232A5024411219 @default.
• W1969863232 hasAuthorship W1969863232A5033126095 @default.
• W1969863232 hasAuthorship W1969863232A5058863432 @default.
• W1969863232 hasConcept C101152365 @default.
• W1969863232 hasConcept C104317684 @default.
• W1969863232 hasConcept C106289968 @default.
• W1969863232 hasConcept C119157956 @default.
• W1969863232 hasConcept C11960822 @default.
• W1969863232 hasConcept C162356407 @default.
• W1969863232 hasConcept C170552419 @default.
• W1969863232 hasConcept C185592680 @default.
• W1969863232 hasConcept C2779933727 @default.
• W1969863232 hasConcept C27826464 @default.
• W1969863232 hasConcept C31827203 @default.
• W1969863232 hasConcept C43617362 @default.
• W1969863232 hasConcept C53345823 @default.
• W1969863232 hasConcept C552990157 @default.
• W1969863232 hasConcept C55493867 @default.
• W1969863232 hasConcept C64927066 @default.
• W1969863232 hasConceptScore W1969863232C101152365 @default.
• W1969863232 hasConceptScore W1969863232C104317684 @default.
• W1969863232 hasConceptScore W1969863232C106289968 @default.
• W1969863232 hasConceptScore W1969863232C119157956 @default.
• W1969863232 hasConceptScore W1969863232C11960822 @default.
• W1969863232 hasConceptScore W1969863232C162356407 @default.
• W1969863232 hasConceptScore W1969863232C170552419 @default.
• W1969863232 hasConceptScore W1969863232C185592680 @default.
• W1969863232 hasConceptScore W1969863232C2779933727 @default.
• W1969863232 hasConceptScore W1969863232C27826464 @default.
• W1969863232 hasConceptScore W1969863232C31827203 @default.
• W1969863232 hasConceptScore W1969863232C43617362 @default.
• W1969863232 hasConceptScore W1969863232C53345823 @default.
• W1969863232 hasConceptScore W1969863232C552990157 @default.
• W1969863232 hasConceptScore W1969863232C55493867 @default.
• W1969863232 hasConceptScore W1969863232C64927066 @default.
• W1969863232 hasIssue "6" @default.

• next