Matches in SemOpenAlex for { <https://semopenalex.org/work/W1969863995> ?p ?o ?g. }
- W1969863995 abstract "By the modification of acidic amino-acid residues with Woodward's reagent K (N-ethyl-5-phenylisoxazolium-3'-sulfonate), the activity of sphingomyelinase of Bacillus cereus was decreased by 80-90%. Also, the reduction of Cys residues in the sphingomyelinase molecule by dithiothreitol caused a drastic decrease in enzymatic activity, whereas the sphingomyelinase activity was not affected by treatment with p-chloromercuribenzenesulfonic acid. Actually, no inactivation of sphingomyelinase activity was observed after selective modification of basic amino-acid residues such as Lys, His and Arg, and of the uncharged amino-acid residues Ser and Thr. The treatment of the sphingomyelinase molecule with Woodward's reagent K or dithiothreitol also brought about the inhibition of the specific adsorption of sphingomyelinase toward intact erythrocyte membranes. However, the extent of inhibition in the enzyme adsorption, 20-50%, was less than that observed in the sphingomyelinase activity. These results suggest that acidic amino-acid residues, such as Asp and Glu, in the sphingomyelinase molecule are involved in the catalytic sites and the adsorptive sites. Apparently, the disruption of disulfide linkage in the sphingomyelinase molecule by dithiothreitol destabilized its structure, resulting in a drastic decrease in sphingomyelin-hydrolyzing activity and specific adsorption of sphingomyelinase towards erythrocyte membranes." @default.
- W1969863995 created "2016-06-24" @default.
- W1969863995 creator A5029449740 @default.
- W1969863995 creator A5030519900 @default.
- W1969863995 creator A5071914412 @default.
- W1969863995 creator A5087301725 @default.
- W1969863995 creator A5088849244 @default.
- W1969863995 date "1993-11-01" @default.
- W1969863995 modified "2023-09-24" @default.
- W1969863995 title "The role of acidic amino-acid residues in catalytic and adsorptive sites of Bacillus cereus spingomyelinase" @default.
- W1969863995 cites W127841036 @default.
- W1969863995 cites W138546028 @default.
- W1969863995 cites W1515339194 @default.
- W1969863995 cites W1522266198 @default.
- W1969863995 cites W1538810654 @default.
- W1969863995 cites W1554197921 @default.
- W1969863995 cites W1584598819 @default.
- W1969863995 cites W1745969379 @default.
- W1969863995 cites W1775749144 @default.
- W1969863995 cites W1809748578 @default.
- W1969863995 cites W1823302164 @default.
- W1969863995 cites W1861288953 @default.
- W1969863995 cites W1876330616 @default.
- W1969863995 cites W1972185778 @default.
- W1969863995 cites W1978783787 @default.
- W1969863995 cites W1982789493 @default.
- W1969863995 cites W1983887481 @default.
- W1969863995 cites W1985535231 @default.
- W1969863995 cites W1987753081 @default.
- W1969863995 cites W1994238274 @default.
- W1969863995 cites W2006849276 @default.
- W1969863995 cites W2013368806 @default.
- W1969863995 cites W2014321075 @default.
- W1969863995 cites W2014992233 @default.
- W1969863995 cites W2020190145 @default.
- W1969863995 cites W2037684843 @default.
- W1969863995 cites W2040083134 @default.
- W1969863995 cites W2046646476 @default.
- W1969863995 cites W2049777280 @default.
- W1969863995 cites W2051689433 @default.
- W1969863995 cites W2073505302 @default.
- W1969863995 cites W2077483651 @default.
- W1969863995 cites W2079501852 @default.
- W1969863995 cites W2079943317 @default.
- W1969863995 cites W2092904207 @default.
- W1969863995 cites W2100837269 @default.
- W1969863995 cites W2110971393 @default.
- W1969863995 cites W2119328842 @default.
- W1969863995 cites W2153435210 @default.
- W1969863995 cites W2157364245 @default.
- W1969863995 cites W2160041699 @default.
- W1969863995 cites W2169679742 @default.
- W1969863995 cites W2324915902 @default.
- W1969863995 cites W258376682 @default.
- W1969863995 doi "https://doi.org/10.1016/0167-4838(93)90039-t" @default.
- W1969863995 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8218395" @default.
- W1969863995 hasPublicationYear "1993" @default.
- W1969863995 type Work @default.
- W1969863995 sameAs 1969863995 @default.
- W1969863995 citedByCount "12" @default.
- W1969863995 countsByYear W19698639952012 @default.
- W1969863995 countsByYear W19698639952014 @default.
- W1969863995 countsByYear W19698639952016 @default.
- W1969863995 crossrefType "journal-article" @default.
- W1969863995 hasAuthorship W1969863995A5029449740 @default.
- W1969863995 hasAuthorship W1969863995A5030519900 @default.
- W1969863995 hasAuthorship W1969863995A5071914412 @default.
- W1969863995 hasAuthorship W1969863995A5087301725 @default.
- W1969863995 hasAuthorship W1969863995A5088849244 @default.
- W1969863995 hasConcept C178790620 @default.
- W1969863995 hasConcept C181199279 @default.
- W1969863995 hasConcept C185592680 @default.
- W1969863995 hasConcept C2777118263 @default.
- W1969863995 hasConcept C2777824588 @default.
- W1969863995 hasConcept C2779201268 @default.
- W1969863995 hasConcept C2781070647 @default.
- W1969863995 hasConcept C2781457462 @default.
- W1969863995 hasConcept C2910051704 @default.
- W1969863995 hasConcept C40875361 @default.
- W1969863995 hasConcept C41625074 @default.
- W1969863995 hasConcept C515207424 @default.
- W1969863995 hasConcept C523546767 @default.
- W1969863995 hasConcept C54355233 @default.
- W1969863995 hasConcept C55493867 @default.
- W1969863995 hasConcept C61322309 @default.
- W1969863995 hasConcept C86803240 @default.
- W1969863995 hasConceptScore W1969863995C178790620 @default.
- W1969863995 hasConceptScore W1969863995C181199279 @default.
- W1969863995 hasConceptScore W1969863995C185592680 @default.
- W1969863995 hasConceptScore W1969863995C2777118263 @default.
- W1969863995 hasConceptScore W1969863995C2777824588 @default.
- W1969863995 hasConceptScore W1969863995C2779201268 @default.
- W1969863995 hasConceptScore W1969863995C2781070647 @default.
- W1969863995 hasConceptScore W1969863995C2781457462 @default.
- W1969863995 hasConceptScore W1969863995C2910051704 @default.
- W1969863995 hasConceptScore W1969863995C40875361 @default.
- W1969863995 hasConceptScore W1969863995C41625074 @default.
- W1969863995 hasConceptScore W1969863995C515207424 @default.
- W1969863995 hasConceptScore W1969863995C523546767 @default.
- W1969863995 hasConceptScore W1969863995C54355233 @default.