Matches in SemOpenAlex for { <https://semopenalex.org/work/W1969917966> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W1969917966 endingPage "45" @default.
- W1969917966 startingPage "34" @default.
- W1969917966 abstract "A quantitative proteomics workflow was implemented that provides extended plasma protein coverage by extensive protein depletion in combination with the sensitivity and breadth of analysis of two-dimensional LC-MS/MS shotgun analysis. Abundant proteins were depleted by a two-stage process using IgY and Supermix depletion columns in series. Samples are then extensively fractionated by two-dimensional chromatography with fractions directly deposited onto MALDI plates. Decoupling sample fractionation from mass spectrometry facilitates a targeted MS/MS precursor selection strategy that maximizes measurement of a consistent set of peptides across experiments. Multiplexed stable isotope labeling provides quantification relative to a common reference sample and ensures an identical set of peptides measured in the set of samples (set of eight) combined in a single experiment. The more extensive protein depletion provided by the addition of the Supermix column did not compromise overall reproducibility of the measurements or the ability to reliably detect changes in protein levels between samples. The implementation of this workflow is presented for a case study aimed at generating molecular signatures for prediction of first heart attack." @default.
- W1969917966 created "2016-06-24" @default.
- W1969917966 creator A5000461546 @default.
- W1969917966 creator A5001008409 @default.
- W1969917966 creator A5010200052 @default.
- W1969917966 creator A5012889242 @default.
- W1969917966 creator A5044445398 @default.
- W1969917966 creator A5062117428 @default.
- W1969917966 creator A5068077627 @default.
- W1969917966 creator A5068708440 @default.
- W1969917966 creator A5070862532 @default.
- W1969917966 creator A5078222979 @default.
- W1969917966 creator A5081747747 @default.
- W1969917966 creator A5083729859 @default.
- W1969917966 creator A5085854353 @default.
- W1969917966 date "2011-01-07" @default.
- W1969917966 modified "2023-10-13" @default.
- W1969917966 title "Semi-Targeted Plasma Proteomics Discovery Workflow Utilizing Two-Stage Protein Depletion and Off-Line LC−MALDI MS/MS" @default.
- W1969917966 doi "https://doi.org/10.1021/pr100659e" @default.
- W1969917966 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20936781" @default.
- W1969917966 hasPublicationYear "2011" @default.
- W1969917966 type Work @default.
- W1969917966 sameAs 1969917966 @default.
- W1969917966 citedByCount "22" @default.
- W1969917966 countsByYear W19699179662012 @default.
- W1969917966 countsByYear W19699179662013 @default.
- W1969917966 countsByYear W19699179662014 @default.
- W1969917966 countsByYear W19699179662016 @default.
- W1969917966 countsByYear W19699179662017 @default.
- W1969917966 countsByYear W19699179662018 @default.
- W1969917966 countsByYear W19699179662020 @default.
- W1969917966 countsByYear W19699179662021 @default.
- W1969917966 crossrefType "journal-article" @default.
- W1969917966 hasAuthorship W1969917966A5000461546 @default.
- W1969917966 hasAuthorship W1969917966A5001008409 @default.
- W1969917966 hasAuthorship W1969917966A5010200052 @default.
- W1969917966 hasAuthorship W1969917966A5012889242 @default.
- W1969917966 hasAuthorship W1969917966A5044445398 @default.
- W1969917966 hasAuthorship W1969917966A5062117428 @default.
- W1969917966 hasAuthorship W1969917966A5068077627 @default.
- W1969917966 hasAuthorship W1969917966A5068708440 @default.
- W1969917966 hasAuthorship W1969917966A5070862532 @default.
- W1969917966 hasAuthorship W1969917966A5078222979 @default.
- W1969917966 hasAuthorship W1969917966A5081747747 @default.
- W1969917966 hasAuthorship W1969917966A5083729859 @default.
- W1969917966 hasAuthorship W1969917966A5085854353 @default.
- W1969917966 hasConcept C104317684 @default.
- W1969917966 hasConcept C104397665 @default.
- W1969917966 hasConcept C113196181 @default.
- W1969917966 hasConcept C162356407 @default.
- W1969917966 hasConcept C184185385 @default.
- W1969917966 hasConcept C185592680 @default.
- W1969917966 hasConcept C202157981 @default.
- W1969917966 hasConcept C2778576202 @default.
- W1969917966 hasConcept C2781434637 @default.
- W1969917966 hasConcept C31827203 @default.
- W1969917966 hasConcept C43617362 @default.
- W1969917966 hasConcept C46111723 @default.
- W1969917966 hasConcept C55493867 @default.
- W1969917966 hasConcept C68289359 @default.
- W1969917966 hasConcept C80311884 @default.
- W1969917966 hasConcept C97428945 @default.
- W1969917966 hasConceptScore W1969917966C104317684 @default.
- W1969917966 hasConceptScore W1969917966C104397665 @default.
- W1969917966 hasConceptScore W1969917966C113196181 @default.
- W1969917966 hasConceptScore W1969917966C162356407 @default.
- W1969917966 hasConceptScore W1969917966C184185385 @default.
- W1969917966 hasConceptScore W1969917966C185592680 @default.
- W1969917966 hasConceptScore W1969917966C202157981 @default.
- W1969917966 hasConceptScore W1969917966C2778576202 @default.
- W1969917966 hasConceptScore W1969917966C2781434637 @default.
- W1969917966 hasConceptScore W1969917966C31827203 @default.
- W1969917966 hasConceptScore W1969917966C43617362 @default.
- W1969917966 hasConceptScore W1969917966C46111723 @default.
- W1969917966 hasConceptScore W1969917966C55493867 @default.
- W1969917966 hasConceptScore W1969917966C68289359 @default.
- W1969917966 hasConceptScore W1969917966C80311884 @default.
- W1969917966 hasConceptScore W1969917966C97428945 @default.
- W1969917966 hasIssue "1" @default.
- W1969917966 hasLocation W19699179661 @default.
- W1969917966 hasLocation W19699179662 @default.
- W1969917966 hasOpenAccess W1969917966 @default.
- W1969917966 hasPrimaryLocation W19699179661 @default.
- W1969917966 hasRelatedWork W1978702876 @default.
- W1969917966 hasRelatedWork W2067982561 @default.
- W1969917966 hasRelatedWork W2082424310 @default.
- W1969917966 hasRelatedWork W2157839186 @default.
- W1969917966 hasRelatedWork W2163021396 @default.
- W1969917966 hasRelatedWork W2402908082 @default.
- W1969917966 hasRelatedWork W2436016506 @default.
- W1969917966 hasRelatedWork W2488667560 @default.
- W1969917966 hasRelatedWork W2570472928 @default.
- W1969917966 hasRelatedWork W2593885388 @default.
- W1969917966 hasVolume "10" @default.
- W1969917966 isParatext "false" @default.
- W1969917966 isRetracted "false" @default.
- W1969917966 magId "1969917966" @default.
- W1969917966 workType "article" @default.