FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1969968451> ?p ?o ?g. }

• W1969968451 endingPage "567" @default.
• W1969968451 startingPage "560" @default.
• W1969968451 abstract "Dengue virus infects millions of people worldwide, and there is no vaccine or anti-dengue therapeutic available. Antimicrobial peptides have been shown to possess effective antiviral activity against various viruses. One of the main limitations of developing these peptides as potent antiviral drugs is the high cost of production. In this study, high yield production of biologically active plectasin peptide was inexpensively achieved by producing tandem plectasin peptides as inclusion bodies in E. coli. Antiviral activity of the recombinant peptide towards dengue serotype-2 NS2B-NS3 protease (DENV2 NS2B-NS3pro) was assessed as a target to inhibit dengue virus replication in Vero cells. Single units of recombinant plectasin were collected after applying consecutive steps of refolding, cleaving by Factor Xa, and nickel column purification to obtain recombinant proteins of high purity. The maximal nontoxic dose (MNTD) of the recombinant peptide against Vero cells was 20 μM (100 μg/mL). The reaction velocity of DENV2 NS2B-NS3pro decreased significantly after increasing concentrations of recombinant plectasin were applied to the reaction mixture. Plectasin peptide noncompetitively inhibited DENV2 NS2B-NS3pro at Ki value of 5.03±0.98 μM. The percentage of viral inhibition was more than 80% at the MNTD value of plectasin. In this study, biologically active recombinant plectasin which was able to inhibit dengue protease and viral replication in Vero cells was successfully produced in E. coli in a time- and cost- effective method. These findings are potentially important in the development of potent therapeutics against dengue infection." @default.
• W1969968451 created "2016-06-24" @default.
• W1969968451 creator A5006392712 @default.
• W1969968451 creator A5010681619 @default.
• W1969968451 creator A5025557536 @default.
• W1969968451 creator A5071652002 @default.
• W1969968451 creator A5078077536 @default.
• W1969968451 date "2013-11-01" @default.
• W1969968451 modified "2023-10-16" @default.
• W1969968451 title "Antiviral Cationic Peptides as a Strategy for Innovation in Global Health Therapeutics for Dengue Virus: High Yield Production of the Biologically Active Recombinant Plectasin Peptide" @default.
• W1969968451 cites W1963722730 @default.
• W1969968451 cites W1965814620 @default.
• W1969968451 cites W1970169619 @default.
• W1969968451 cites W1975863174 @default.
• W1969968451 cites W1979404035 @default.
• W1969968451 cites W2000530956 @default.
• W1969968451 cites W2006863478 @default.
• W1969968451 cites W2013674343 @default.
• W1969968451 cites W2021236720 @default.
• W1969968451 cites W2022668283 @default.
• W1969968451 cites W2026899492 @default.
• W1969968451 cites W2028064829 @default.
• W1969968451 cites W2037831589 @default.
• W1969968451 cites W2042819115 @default.
• W1969968451 cites W2045211613 @default.
• W1969968451 cites W2046997318 @default.
• W1969968451 cites W2051034707 @default.
• W1969968451 cites W2053125868 @default.
• W1969968451 cites W2077098425 @default.
• W1969968451 cites W2077332176 @default.
• W1969968451 cites W2079949399 @default.
• W1969968451 cites W2089210389 @default.
• W1969968451 cites W2091265811 @default.
• W1969968451 cites W2098687402 @default.
• W1969968451 cites W2103544344 @default.
• W1969968451 cites W2111089744 @default.
• W1969968451 cites W2123850573 @default.
• W1969968451 cites W2124547357 @default.
• W1969968451 cites W2125126375 @default.
• W1969968451 cites W2131462376 @default.
• W1969968451 cites W2132935126 @default.
• W1969968451 cites W2137995709 @default.
• W1969968451 cites W2138717574 @default.
• W1969968451 cites W2157477641 @default.
• W1969968451 cites W2165613183 @default.
• W1969968451 doi "https://doi.org/10.1089/omi.2013.0056" @default.
• W1969968451 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3814901" @default.
• W1969968451 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24044366" @default.
• W1969968451 hasPublicationYear "2013" @default.
• W1969968451 type Work @default.
• W1969968451 sameAs 1969968451 @default.
• W1969968451 citedByCount "31" @default.
• W1969968451 countsByYear W19699684512014 @default.
• W1969968451 countsByYear W19699684512015 @default.
• W1969968451 countsByYear W19699684512016 @default.
• W1969968451 countsByYear W19699684512017 @default.
• W1969968451 countsByYear W19699684512018 @default.
• W1969968451 countsByYear W19699684512019 @default.
• W1969968451 countsByYear W19699684512020 @default.
• W1969968451 countsByYear W19699684512021 @default.
• W1969968451 countsByYear W19699684512022 @default.
• W1969968451 countsByYear W19699684512023 @default.
• W1969968451 crossrefType "journal-article" @default.
• W1969968451 hasAuthorship W1969968451A5006392712 @default.
• W1969968451 hasAuthorship W1969968451A5010681619 @default.
• W1969968451 hasAuthorship W1969968451A5025557536 @default.
• W1969968451 hasAuthorship W1969968451A5071652002 @default.
• W1969968451 hasAuthorship W1969968451A5078077536 @default.
• W1969968451 hasBestOaLocation W19699684512 @default.
• W1969968451 hasConcept C104317684 @default.
• W1969968451 hasConcept C132379496 @default.
• W1969968451 hasConcept C159047783 @default.
• W1969968451 hasConcept C181199279 @default.
• W1969968451 hasConcept C203750385 @default.
• W1969968451 hasConcept C2522874641 @default.
• W1969968451 hasConcept C2776408679 @default.
• W1969968451 hasConcept C2776714187 @default.
• W1969968451 hasConcept C2779281246 @default.
• W1969968451 hasConcept C2779635636 @default.
• W1969968451 hasConcept C40767141 @default.
• W1969968451 hasConcept C533803919 @default.
• W1969968451 hasConcept C55493867 @default.
• W1969968451 hasConcept C86803240 @default.
• W1969968451 hasConcept C89138240 @default.
• W1969968451 hasConcept C89423630 @default.
• W1969968451 hasConceptScore W1969968451C104317684 @default.
• W1969968451 hasConceptScore W1969968451C132379496 @default.
• W1969968451 hasConceptScore W1969968451C159047783 @default.
• W1969968451 hasConceptScore W1969968451C181199279 @default.
• W1969968451 hasConceptScore W1969968451C203750385 @default.
• W1969968451 hasConceptScore W1969968451C2522874641 @default.
• W1969968451 hasConceptScore W1969968451C2776408679 @default.
• W1969968451 hasConceptScore W1969968451C2776714187 @default.
• W1969968451 hasConceptScore W1969968451C2779281246 @default.
• W1969968451 hasConceptScore W1969968451C2779635636 @default.
• W1969968451 hasConceptScore W1969968451C40767141 @default.
• W1969968451 hasConceptScore W1969968451C533803919 @default.
• W1969968451 hasConceptScore W1969968451C55493867 @default.

• next