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- W1970049058 abstract "A study has been made with pig red blood cells of the activation of the sodium pump by internal and external cations. Cell Na and K concentrations were altered using a PCMBS cation loading procedure. The procedure was characterised for resultant ionic conditions, maintenance of ATP levels and fragility. The activation of the sodium pump by external K was measured in cells suspended in choline (Na-free) solutions. External Cs was used as a substitute for K and elicited lower rates of pump activity. Both the Vmax and apparent Km for 42K influx and 134Cs influx increased as internal Na concentration was raised (within the non-saturating range). Vmax/apparent Km ratios for cation influx were constant. Raising external Cs concentration exerted a similar influence on pump activation by internal Na: both the maximum pump velocity and the apparent Na-site dissociation constant (K'Na) increased. The results provide evidence for a transmembrane connection between cation binding sites on opposite faces of the membrane and are consistent with a consecutive model for the sodium pump in pig red blood cells." @default.
- W1970049058 created "2016-06-24" @default.
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- W1970049058 date "1985-05-01" @default.
- W1970049058 modified "2023-09-27" @default.
- W1970049058 title "The activation of the sodium pump in pig red blood cells by internal and external cations" @default.
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- W1970049058 doi "https://doi.org/10.1016/0167-4889(85)90170-3" @default.
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