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- W1970087348 abstract "Abstract A sensitive procedure for the determination of oestradiol in small plasma samples by competitive-binding to uterine supernatant preparations has been described. Although preparations from other species were examined, uterine supernatants from New Zealand White rabbits (2–2.5 kg) were preferred because of the low dissociation constant ( K d ) of the equilibrium.The quality of these preparations was dependent on the state of maturity of the animal and was not affected by previous mating. The assay, which was carried out at 30°C, reached equilibrium within 30 min yet gave high precision and low blanks. The method has been applied to human peripheral plasma throughout the menstrual cycle and to rat ovarian plasma throughout the oestrous cycle." @default.
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- W1970087348 date "1971-02-01" @default.
- W1970087348 modified "2023-09-26" @default.
- W1970087348 title "Assay of plasma oestradiol by competitive-binding to uterine supernatant protein" @default.
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- W1970087348 doi "https://doi.org/10.1016/0022-4731(71)90003-3" @default.
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