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• W1970102211 abstract "Zinc, copper and cadmium are important environmental contaminants and differences in purinergic and cholinergic systems of invertebrates have been described when compared to characteristics of these signaling systems in vertebrates. Here we evaluate the effect in vitro of these metals on the ATPase, 5′-nucleotidase and cholinesterase (ChE) activities in the digestive gland of Helix aspersa. Zinc (500 and 1000 μM) promoted a significant decrease in 5′-nucleotidase activity. However, it did not induce changes in ATP hydrolysis. Copper (25 and 50 μM), inhibited significantly ATPase activity, but did not alter 5′-nucleotidase when compared to control (no metal added). In relation to effects of cadmium, an inhibitory effect on ATP hydrolysis has been observed at concentrations of 100, 500 and 1000 μM and a similar decrease of AMP hydrolysis was observed at 500 and 1000 μM. However, there were no significant changes in ChE activity from homogenates of the digestive gland of H. aspersa for all metals tested. This study demonstrated that zinc, cadmium and copper affect ATPase and 5′-nucleotidase in digestive gland, but not ChE, suggesting that the purinergic system may be a target related to toxicity induced by these metals and a possible indicator of biological impact of exposure to these contaminants." @default.
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• W1970102211 date "2006-07-01" @default.
• W1970102211 modified "2023-10-16" @default.
• W1970102211 title "In vitro exposure of heavy metals on nucleotidase and cholinesterase activities from the digestive gland of Helix aspersa" @default.
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• W1970102211 doi "https://doi.org/10.1016/j.cbpc.2006.03.005" @default.
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