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- W1970140322 abstract "Abstract We compared the ability of dialysed fetal bovine serum and of combinations of purified growth-promoting factors such as insulin, epidermal growth factor (EGF), vasopressin, fibroblast-derived growth factor and antitubulin agents to stimulate DNA synthesis in 3T3 cells maintained in the absence or presence of inorganic phosphate (P i ). When DNA synthesis was stimulated by serum in the absence of P i the level induced was 70% of that observed in P i -containing medium. In contrast, combinations of growth-promoting factors in the absence of P i stimulated less than 8% of the DNA synthesis which they induced in complete medium. Addition of as little as 50 μM P i fully restored the ability of the factors to stimulate DNA synthesis. Cells stimulated by purified mitogens in the absence of P i became blocked in early G1, and for up to 48 h the block was reversible by readdition of p i . The effectiveness of dialysed serum to stimulate DNA in the absence of P i suggest that dialysed serum might contain a component capable of supplying P i to support DNA synthesis. Indeed, delipidization of serum by solvent extraction resulted in loss of ability to stimulate DNA synthesis in the absence of p i , but delipidized serum stimulated DNA synthesis virtually, as well as dialysed serum in the presence of P i . Previous conclusions suggesting that P i is not essential for DNA synthesis appear to require re-evaluation." @default.
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- W1970140322 date "1982-06-01" @default.
- W1970140322 modified "2023-09-27" @default.
- W1970140322 title "Inorganic phosphate is necessary for the stimulation of DNA synthesis in swiss 3T3 cells by pure mitogenic agents" @default.
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- W1970140322 doi "https://doi.org/10.1016/0014-4827(82)90273-7" @default.
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