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• W1970147605 abstract "Abstract Illumination of intact pea chloroplasts results in both the pH activation and the thiol modulation of the reversible protonmotive ATPase, or CF 0 -CF 1 (Mills, J.D., Mitchell, P. and Schurmann, P. (1980) FEBS Lett. 112, 173–177). We have studied the reversal of the activation and thiol-modulation processes that occurs when illuminated chloroplasts are darkened. (1) Methyl viologen is shown to prevent light-dependent thiol modulation of CF 0 -CF 1 in intact chloroplasts. Studies using methyl viologen indicate that the decline in ATPase activity of intact chloroplasts in the dark is due both to pH deactivation and thiol demodulation of CF 0 -CF 1 . Reillumination in the presence of methyl viologen reactivates CF 0 -CF 1 complexes that have undergone pH deactivation, but does not allow thiol modulation to occur. (2) At 20°C, both pH deactivation and thiol demodulation of CF 0 -CF 1 are complete within 10 min darkness. At 3.5°C, both processes are retarded, and require more than 30 min for completion. (3) Lysis of intact chloroplasts greatly retards the thiol demodulation of CF 0 -CF 1 . In the dark, addition of oxidants to lysed chloroplasts promotes thiol demodulation. Under these conditions, thioredoxin behaves as a weak oxidant, but glutathione has very little effect. Illumination of intact chloroplasts followed by rapid lysis and storage on ice stabilises the pH-activated, thiol-modulated state of CF 0 -CF 1 for extended periods in the dark and increases the time required for thiol demodulation to well over 2 h. This method may prove to be useful for preparing thiol-modulated chloroplasts without the need to add or remove exogenous thiols. The results suggest that an oxidative system exists in the stroma which actively reverses thiol modulation of CF 0 -CF 1 in the dark. Such a system would be expected if thiol modulation of CF 0 -CF 1 were an important physiological process regulating reversible ATPase activity in vivo." @default.
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• W1970147605 date "1982-01-01" @default.
• W1970147605 modified "2023-09-23" @default.
• W1970147605 title "Modulation of coupling factor ATPase activity in intact chloroplasts. Reversal of thiol modulation in the dark" @default.
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• W1970147605 doi "https://doi.org/10.1016/0005-2728(82)90257-2" @default.
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