FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1970168006> ?p ?o ?g. }

• W1970168006 endingPage "572" @default.
• W1970168006 startingPage "564" @default.
• W1970168006 abstract "We have developed a novel Escherichia coli cell surface display system by employing PgsA as an anchoring motif. In our display system, C-terminal fusion to PgsA anchor protein from Bacillus subtilis was used. The enzymes selected for display were α-amylase (AmyA) from Streptococcus bovis 148 and lipase B (CALB) from Candida antarctica. The molecular mass values of AmyA and CALB are approximately 77 and 34 kDa, respectively. The enzymes were displayed on the surface as a fusion protein with a FLAG peptide tag at the C terminus. Both the PgsA-AmyA-FLAG and PgsA-CALB-FLAG fusion proteins were shown to be displayed by immunofluorescence labeling using anti-FLAG antibody. The displayed enzymes were active forms, and AmyA and CALB activities reached 990 U/g (dry cell weight) and 4.6 U/g (dry cell weight), respectively. AmyA-displaying E. coli cells grew utilizing cornstarch as the sole carbon source, while CALB-displaying E. coli cells catalyzed enantioselective transesterification, indicating that they are effective whole-cell biocatalysts. Since a target enzyme with a size of 77 kDa and an industrially useful lipase have been successfully displayed on the cell surface of E. coli for the first time, PgsA protein is probably a useful anchoring motif to display various enzymes." @default.
• W1970168006 created "2016-06-24" @default.
• W1970168006 creator A5005714024 @default.
• W1970168006 creator A5014256354 @default.
• W1970168006 creator A5034328544 @default.
• W1970168006 creator A5036849342 @default.
• W1970168006 creator A5048418245 @default.
• W1970168006 creator A5068551563 @default.
• W1970168006 creator A5075554907 @default.
• W1970168006 creator A5088547972 @default.
• W1970168006 date "2005-08-20" @default.
• W1970168006 modified "2023-09-27" @default.
• W1970168006 title "Display of active enzymes on the cell surface of Escherichia coli using PgsA anchor protein and their application to bioconversion" @default.
• W1970168006 cites W1580355885 @default.
• W1970168006 cites W1581860251 @default.
• W1970168006 cites W1599065114 @default.
• W1970168006 cites W1962450832 @default.
• W1970168006 cites W1968834637 @default.
• W1970168006 cites W1973101229 @default.
• W1970168006 cites W1985935760 @default.
• W1970168006 cites W1991170357 @default.
• W1970168006 cites W1994696334 @default.
• W1970168006 cites W2016789574 @default.
• W1970168006 cites W2027595747 @default.
• W1970168006 cites W2033495093 @default.
• W1970168006 cites W2043587098 @default.
• W1970168006 cites W2047590567 @default.
• W1970168006 cites W2050466522 @default.
• W1970168006 cites W2051895530 @default.
• W1970168006 cites W2058907741 @default.
• W1970168006 cites W2070082076 @default.
• W1970168006 cites W2070849872 @default.
• W1970168006 cites W2070943709 @default.
• W1970168006 cites W2084934720 @default.
• W1970168006 cites W2100850148 @default.
• W1970168006 cites W2119279742 @default.
• W1970168006 cites W2138879032 @default.
• W1970168006 cites W2139444044 @default.
• W1970168006 cites W2155498712 @default.
• W1970168006 doi "https://doi.org/10.1007/s00253-005-0111-x" @default.
• W1970168006 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16133338" @default.
• W1970168006 hasPublicationYear "2005" @default.
• W1970168006 type Work @default.
• W1970168006 sameAs 1970168006 @default.
• W1970168006 citedByCount "86" @default.
• W1970168006 countsByYear W19701680062012 @default.
• W1970168006 countsByYear W19701680062013 @default.
• W1970168006 countsByYear W19701680062014 @default.
• W1970168006 countsByYear W19701680062015 @default.
• W1970168006 countsByYear W19701680062016 @default.
• W1970168006 countsByYear W19701680062017 @default.
• W1970168006 countsByYear W19701680062018 @default.
• W1970168006 countsByYear W19701680062019 @default.
• W1970168006 countsByYear W19701680062021 @default.
• W1970168006 countsByYear W19701680062022 @default.
• W1970168006 countsByYear W19701680062023 @default.
• W1970168006 crossrefType "journal-article" @default.
• W1970168006 hasAuthorship W1970168006A5005714024 @default.
• W1970168006 hasAuthorship W1970168006A5014256354 @default.
• W1970168006 hasAuthorship W1970168006A5034328544 @default.
• W1970168006 hasAuthorship W1970168006A5036849342 @default.
• W1970168006 hasAuthorship W1970168006A5048418245 @default.
• W1970168006 hasAuthorship W1970168006A5068551563 @default.
• W1970168006 hasAuthorship W1970168006A5075554907 @default.
• W1970168006 hasAuthorship W1970168006A5088547972 @default.
• W1970168006 hasBestOaLocation W19701680061 @default.
• W1970168006 hasConcept C100544194 @default.
• W1970168006 hasConcept C104317684 @default.
• W1970168006 hasConcept C123894998 @default.
• W1970168006 hasConcept C181199279 @default.
• W1970168006 hasConcept C185592680 @default.
• W1970168006 hasConcept C2779542420 @default.
• W1970168006 hasConcept C2779697368 @default.
• W1970168006 hasConcept C2780074830 @default.
• W1970168006 hasConcept C40767141 @default.
• W1970168006 hasConcept C547475151 @default.
• W1970168006 hasConcept C55493867 @default.
• W1970168006 hasConceptScore W1970168006C100544194 @default.
• W1970168006 hasConceptScore W1970168006C104317684 @default.
• W1970168006 hasConceptScore W1970168006C123894998 @default.
• W1970168006 hasConceptScore W1970168006C181199279 @default.
• W1970168006 hasConceptScore W1970168006C185592680 @default.
• W1970168006 hasConceptScore W1970168006C2779542420 @default.
• W1970168006 hasConceptScore W1970168006C2779697368 @default.
• W1970168006 hasConceptScore W1970168006C2780074830 @default.
• W1970168006 hasConceptScore W1970168006C40767141 @default.
• W1970168006 hasConceptScore W1970168006C547475151 @default.
• W1970168006 hasConceptScore W1970168006C55493867 @default.
• W1970168006 hasIssue "5" @default.
• W1970168006 hasLocation W19701680061 @default.
• W1970168006 hasLocation W19701680062 @default.
• W1970168006 hasOpenAccess W1970168006 @default.
• W1970168006 hasPrimaryLocation W19701680061 @default.
• W1970168006 hasRelatedWork W1717544097 @default.
• W1970168006 hasRelatedWork W1972665833 @default.
• W1970168006 hasRelatedWork W1984056175 @default.
• W1970168006 hasRelatedWork W2019087025 @default.
• W1970168006 hasRelatedWork W2025398173 @default.

• next