FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1970191962> ?p ?o ?g. }

• W1970191962 abstract "The Thymidine kinase (Tk) mutants generated from the widely used L5178Y mouse lymphoma assay fall into two categories, small colony and large colony. Cells from the large colonies grow at a normal rate while cells from the small colonies grow slower than normal. The relative proportion of large and small colonies after mutagen treatment is associated with a mutagen's ability to induce point mutations and/or chromosomal mutations. The molecular distinction between large and small colony mutants, however, is not clear.To gain insights into the underlying mechanisms responsible for the mutant colony phenotype, microarray gene expression analysis was carried out on 4 small and 4 large colony Tk mutant samples. NCTR-fabricated long-oligonucleotide microarrays of 20,000 mouse genes were used in a two-color reference design experiment. The data were analyzed within ArrayTrack software that was developed at the NCTR. Principal component analysis and hierarchical clustering of the gene expression profiles showed that the samples were clearly separated into two groups based on their colony size phenotypes. The Welch T-test was used for determining significant changes in gene expression between the large and small colony groups and 90 genes whose expression was significantly altered were identified (p < 0.01; fold change > 1.5). Using Ingenuity Pathways Analysis (IPA), 50 out of the 90 significant genes were found in the IPA database and mapped to four networks associated with cell growth. Eleven percent of the 90 significant genes were located on chromosome 11 where the Tk gene resides while only 5.6% of the genes on the microarrays mapped to chromosome 11. All of the chromosome 11 significant genes were expressed at a higher level in the small colony mutants compared to the large colony mutants. Also, most of the significant genes located on chromosome 11 were disproportionally concentrated on the distal end of chromosome 11 where the Tk mutations occurred.The results indicate that microarray analysis can define cellular phenotypes and identify genes that are related to the colony size phenotypes. The findings suggest that genes in the DNA segment altered by the Tk mutations were significantly up-regulated in the small colony mutants, but not in the large colony mutants, leading to differential expression of a set of growth regulation genes that are related to cell apoptosis and other cellular functions related to the restriction of cell growth." @default.
• W1970191962 created "2016-06-24" @default.
• W1970191962 creator A5001024551 @default.
• W1970191962 creator A5015628950 @default.
• W1970191962 creator A5018882292 @default.
• W1970191962 creator A5068886380 @default.
• W1970191962 creator A5078091393 @default.
• W1970191962 creator A5078108644 @default.
• W1970191962 date "2006-09-01" @default.
• W1970191962 modified "2023-10-18" @default.
• W1970191962 title "Microarray analysis distinguishes differential gene expression patterns from large and small colony Thymidine kinase mutants of L5178Y mouse lymphoma cells" @default.
• W1970191962 cites W1500465676 @default.
• W1970191962 cites W1564298331 @default.
• W1970191962 cites W1565383288 @default.
• W1970191962 cites W1982061874 @default.
• W1970191962 cites W1990281533 @default.
• W1970191962 cites W1994902349 @default.
• W1970191962 cites W1995672426 @default.
• W1970191962 cites W2006316372 @default.
• W1970191962 cites W2008812480 @default.
• W1970191962 cites W2022954958 @default.
• W1970191962 cites W2025120938 @default.
• W1970191962 cites W2026936875 @default.
• W1970191962 cites W2029144127 @default.
• W1970191962 cites W2035820068 @default.
• W1970191962 cites W2037220006 @default.
• W1970191962 cites W2037872240 @default.
• W1970191962 cites W2043317995 @default.
• W1970191962 cites W2043792373 @default.
• W1970191962 cites W2046024062 @default.
• W1970191962 cites W2055337311 @default.
• W1970191962 cites W2058298987 @default.
• W1970191962 cites W2059802429 @default.
• W1970191962 cites W2060783964 @default.
• W1970191962 cites W2062607775 @default.
• W1970191962 cites W2068012172 @default.
• W1970191962 cites W2078597151 @default.
• W1970191962 cites W2082473616 @default.
• W1970191962 cites W2136422121 @default.
• W1970191962 cites W2147955536 @default.
• W1970191962 cites W2168555653 @default.
• W1970191962 cites W2207298758 @default.
• W1970191962 cites W3121489037 @default.
• W1970191962 cites W4236152987 @default.
• W1970191962 doi "https://doi.org/10.1186/1471-2105-7-s2-s9" @default.
• W1970191962 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1683564" @default.
• W1970191962 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17118152" @default.
• W1970191962 hasPublicationYear "2006" @default.
• W1970191962 type Work @default.
• W1970191962 sameAs 1970191962 @default.
• W1970191962 citedByCount "22" @default.
• W1970191962 countsByYear W19701919622012 @default.
• W1970191962 countsByYear W19701919622013 @default.
• W1970191962 countsByYear W19701919622014 @default.
• W1970191962 countsByYear W19701919622015 @default.
• W1970191962 countsByYear W19701919622016 @default.
• W1970191962 countsByYear W19701919622017 @default.
• W1970191962 countsByYear W19701919622018 @default.
• W1970191962 countsByYear W19701919622022 @default.
• W1970191962 crossrefType "journal-article" @default.
• W1970191962 hasAuthorship W1970191962A5001024551 @default.
• W1970191962 hasAuthorship W1970191962A5015628950 @default.
• W1970191962 hasAuthorship W1970191962A5018882292 @default.
• W1970191962 hasAuthorship W1970191962A5068886380 @default.
• W1970191962 hasAuthorship W1970191962A5078091393 @default.
• W1970191962 hasAuthorship W1970191962A5078108644 @default.
• W1970191962 hasBestOaLocation W19701919621 @default.
• W1970191962 hasConcept C104317684 @default.
• W1970191962 hasConcept C127716648 @default.
• W1970191962 hasConcept C143065580 @default.
• W1970191962 hasConcept C150194340 @default.
• W1970191962 hasConcept C153911025 @default.
• W1970191962 hasConcept C18431079 @default.
• W1970191962 hasConcept C186836561 @default.
• W1970191962 hasConcept C2776639595 @default.
• W1970191962 hasConcept C54355233 @default.
• W1970191962 hasConcept C552990157 @default.
• W1970191962 hasConcept C8415881 @default.
• W1970191962 hasConcept C86803240 @default.
• W1970191962 hasConcept C95371953 @default.
• W1970191962 hasConceptScore W1970191962C104317684 @default.
• W1970191962 hasConceptScore W1970191962C127716648 @default.
• W1970191962 hasConceptScore W1970191962C143065580 @default.
• W1970191962 hasConceptScore W1970191962C150194340 @default.
• W1970191962 hasConceptScore W1970191962C153911025 @default.
• W1970191962 hasConceptScore W1970191962C18431079 @default.
• W1970191962 hasConceptScore W1970191962C186836561 @default.
• W1970191962 hasConceptScore W1970191962C2776639595 @default.
• W1970191962 hasConceptScore W1970191962C54355233 @default.
• W1970191962 hasConceptScore W1970191962C552990157 @default.
• W1970191962 hasConceptScore W1970191962C8415881 @default.
• W1970191962 hasConceptScore W1970191962C86803240 @default.
• W1970191962 hasConceptScore W1970191962C95371953 @default.
• W1970191962 hasIssue "S2" @default.
• W1970191962 hasLocation W19701919621 @default.
• W1970191962 hasLocation W19701919622 @default.
• W1970191962 hasLocation W19701919623 @default.
• W1970191962 hasLocation W19701919624 @default.
• W1970191962 hasOpenAccess W1970191962 @default.
• W1970191962 hasPrimaryLocation W19701919621 @default.

• next