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• W1970192695 startingPage "310" @default.
• W1970192695 abstract "Purpose.: To identify whether the kinases that regulate the activity of cation chloride cotransporters (CCC) in other tissues are also expressed in rat and human lenses. Methods.: The expression of with-no-lysine kinase (WNK 1, 3, 4), oxidative stress response kinase 1 (OSR1), and Ste20-like proline alanine rich kinase (SPAK) were determined at either the transcript or protein levels in the rat and human lenses by reverse-transcriptase PCR and/or Western blotting, respectively. Selected kinases were regionally and subcellularly characterized in rat and human lenses. The transparency, wet weight, and tissue morphology of lenses extracted from SPAK knock-out animals was compared with wild-type lenses. Results.: WNK 1, 3, 4, SPAK, and OSR1 were identified at the transcript level in rat lenses and WNK1, 4, SPAK, and OSR1 expression confirmed at the protein level in both rat and human lenses. SPAK and OSR1 were found to associate with membranes as peripheral proteins and exhibited distinct subcellular and region-specific expression profiles throughout the lens. No significant difference in the wet weight of SPAK knock-out lenses was detected relative to wild-type lenses. However, SPAK knock-out lenses showed an increased susceptibility to opacification. Conclusions.: Our results show that the WNK 1, 3, 4, OSR1, and SPAK signaling system known to play a role in regulating the phosphorylation status, and hence activity of the CCCs in other tissues, is also present in the rat and human lenses. The increased susceptibility of SPAK lenses to opacification suggests that disruption of this signaling pathway may compromise the ability of the lens to control its volume, and its ability to maintain its transparency." @default.
• W1970192695 created "2016-06-24" @default.
• W1970192695 creator A5003905457 @default.
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• W1970192695 creator A5088649225 @default.
• W1970192695 date "2014-12-16" @default.
• W1970192695 modified "2023-10-16" @default.
• W1970192695 title "Identification of the WNK-SPAK/OSR1 Signaling Pathway in Rodent and Human Lenses" @default.
• W1970192695 cites W14670389 @default.
• W1970192695 cites W1517779560 @default.
• W1970192695 cites W1530903826 @default.
• W1970192695 cites W1577022703 @default.
• W1970192695 cites W1910989283 @default.
• W1970192695 cites W1963936842 @default.
• W1970192695 cites W1966774376 @default.
• W1970192695 cites W1967010454 @default.
• W1970192695 cites W1967230400 @default.
• W1970192695 cites W1968244525 @default.
• W1970192695 cites W1973047212 @default.
• W1970192695 cites W1975016763 @default.
• W1970192695 cites W1975751336 @default.
• W1970192695 cites W1976554345 @default.
• W1970192695 cites W1977341549 @default.
• W1970192695 cites W1977809782 @default.
• W1970192695 cites W1980244689 @default.
• W1970192695 cites W1982446968 @default.
• W1970192695 cites W1984331164 @default.
• W1970192695 cites W1988257582 @default.
• W1970192695 cites W1988945054 @default.
• W1970192695 cites W1990082022 @default.
• W1970192695 cites W1990788386 @default.
• W1970192695 cites W1993043495 @default.
• W1970192695 cites W1994588831 @default.
• W1970192695 cites W1995517360 @default.
• W1970192695 cites W1997852369 @default.
• W1970192695 cites W2007770885 @default.
• W1970192695 cites W2015439084 @default.
• W1970192695 cites W2015998887 @default.
• W1970192695 cites W2019326144 @default.
• W1970192695 cites W2019496222 @default.
• W1970192695 cites W2020278405 @default.
• W1970192695 cites W2024936138 @default.
• W1970192695 cites W2026495431 @default.
• W1970192695 cites W2027797460 @default.
• W1970192695 cites W2027895266 @default.
• W1970192695 cites W2040331457 @default.
• W1970192695 cites W2041753387 @default.
• W1970192695 cites W2041997615 @default.
• W1970192695 cites W2043451988 @default.
• W1970192695 cites W2047651912 @default.
• W1970192695 cites W2048641604 @default.
• W1970192695 cites W2049549041 @default.
• W1970192695 cites W2052539926 @default.
• W1970192695 cites W2055538694 @default.
• W1970192695 cites W2056466133 @default.
• W1970192695 cites W2063621077 @default.
• W1970192695 cites W2063695292 @default.
• W1970192695 cites W2068431592 @default.
• W1970192695 cites W2070219888 @default.
• W1970192695 cites W2070291201 @default.
• W1970192695 cites W2072982588 @default.
• W1970192695 cites W2076426544 @default.
• W1970192695 cites W2077048490 @default.
• W1970192695 cites W2079156404 @default.
• W1970192695 cites W2080854317 @default.
• W1970192695 cites W2088354913 @default.
• W1970192695 cites W2092544528 @default.
• W1970192695 cites W2093501846 @default.
• W1970192695 cites W2094296416 @default.
• W1970192695 cites W2097798460 @default.
• W1970192695 cites W2099603281 @default.
• W1970192695 cites W2099929505 @default.
• W1970192695 cites W2100755462 @default.
• W1970192695 cites W2107969449 @default.
• W1970192695 cites W2112781680 @default.
• W1970192695 cites W2123263548 @default.
• W1970192695 cites W2129265219 @default.
• W1970192695 cites W2131510524 @default.
• W1970192695 cites W2136605196 @default.
• W1970192695 cites W2142766228 @default.
• W1970192695 cites W2146920291 @default.
• W1970192695 cites W2150380575 @default.
• W1970192695 cites W2150626399 @default.
• W1970192695 cites W2155773980 @default.
• W1970192695 cites W2162642974 @default.
• W1970192695 cites W2163650081 @default.
• W1970192695 cites W2171126674 @default.
• W1970192695 cites W2172151601 @default.
• W1970192695 cites W2275095892 @default.
• W1970192695 doi "https://doi.org/10.1167/iovs.14-15911" @default.
• W1970192695 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4294287" @default.
• W1970192695 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25515571" @default.
• W1970192695 hasPublicationYear "2014" @default.
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