FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1970202326> ?p ?o ?g. }

• W1970202326 endingPage "259" @default.
• W1970202326 startingPage "250" @default.
• W1970202326 abstract "A broad range of genetically-encoded fluorescence biosensors has been developed, allowing the detection of signaling intermediates and metabolites in real time. Many of these biosensors are based on Foerster Resonance Energy Transfer (FRET). The two biosensors of the well-known Venus-flytrap type exemplarily studied in this work are composed of a central sugar binding protein flanked by two green fluorescent protein derivatives, namely ECFP as well as Citrine and EYFP, respectively. In order to evaluate FRET-based biosensors as an in vivo tool for quantitative metabolite analyses, we have thoroughly studied the effects of pH, buffer salts, ionic strength, temperature and several intracellular metabolites on the signal intensity of both biosensors and both fluorescence proteins. Almost all micro-environmental variations led to considerably different FRET signals, because either the fluorescent proteins or the metabolite binding domains were affected by the tested parameters. This resulted not only in altered FRET ratios between the apo state and the saturated state but also in significant shifts of the apparent binding constant. This underlines the necessity of careful controls in order to allow reliable quantitative measurements in vivo." @default.
• W1970202326 created "2016-06-24" @default.
• W1970202326 creator A5014026964 @default.
• W1970202326 creator A5018520418 @default.
• W1970202326 creator A5050958375 @default.
• W1970202326 creator A5059953254 @default.
• W1970202326 creator A5074782661 @default.
• W1970202326 creator A5075919447 @default.
• W1970202326 date "2014-12-01" @default.
• W1970202326 modified "2023-10-07" @default.
• W1970202326 title "An evaluation of genetically encoded FRET-based biosensors for quantitative metabolite analyses in vivo" @default.
• W1970202326 cites W1485557566 @default.
• W1970202326 cites W1489905936 @default.
• W1970202326 cites W1542263802 @default.
• W1970202326 cites W1643784543 @default.
• W1970202326 cites W1941109745 @default.
• W1970202326 cites W1966543898 @default.
• W1970202326 cites W1967626349 @default.
• W1970202326 cites W1969195169 @default.
• W1970202326 cites W1969540020 @default.
• W1970202326 cites W1976136773 @default.
• W1970202326 cites W1977850703 @default.
• W1970202326 cites W1984043202 @default.
• W1970202326 cites W1988824102 @default.
• W1970202326 cites W1991190990 @default.
• W1970202326 cites W1995715600 @default.
• W1970202326 cites W1996744375 @default.
• W1970202326 cites W1999814206 @default.
• W1970202326 cites W2004235182 @default.
• W1970202326 cites W2013412105 @default.
• W1970202326 cites W2015248835 @default.
• W1970202326 cites W2015794466 @default.
• W1970202326 cites W2017923651 @default.
• W1970202326 cites W2018694248 @default.
• W1970202326 cites W2031390995 @default.
• W1970202326 cites W2035726898 @default.
• W1970202326 cites W2055755990 @default.
• W1970202326 cites W2057315336 @default.
• W1970202326 cites W2058393858 @default.
• W1970202326 cites W2062782048 @default.
• W1970202326 cites W2068130092 @default.
• W1970202326 cites W2068447445 @default.
• W1970202326 cites W2070148593 @default.
• W1970202326 cites W2070176786 @default.
• W1970202326 cites W2090212447 @default.
• W1970202326 cites W2094517427 @default.
• W1970202326 cites W2107441982 @default.
• W1970202326 cites W2115014564 @default.
• W1970202326 cites W2129451979 @default.
• W1970202326 cites W2139417020 @default.
• W1970202326 cites W2153535707 @default.
• W1970202326 cites W2159714389 @default.
• W1970202326 cites W2161996750 @default.
• W1970202326 cites W2167042459 @default.
• W1970202326 cites W2168968107 @default.
• W1970202326 cites W2170133970 @default.
• W1970202326 cites W2224116510 @default.
• W1970202326 doi "https://doi.org/10.1016/j.jbiotec.2014.07.007" @default.
• W1970202326 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25107505" @default.
• W1970202326 hasPublicationYear "2014" @default.
• W1970202326 type Work @default.
• W1970202326 sameAs 1970202326 @default.
• W1970202326 citedByCount "29" @default.
• W1970202326 countsByYear W19702023262015 @default.
• W1970202326 countsByYear W19702023262016 @default.
• W1970202326 countsByYear W19702023262017 @default.
• W1970202326 countsByYear W19702023262018 @default.
• W1970202326 countsByYear W19702023262019 @default.
• W1970202326 countsByYear W19702023262020 @default.
• W1970202326 countsByYear W19702023262021 @default.
• W1970202326 countsByYear W19702023262022 @default.
• W1970202326 countsByYear W19702023262023 @default.
• W1970202326 crossrefType "journal-article" @default.
• W1970202326 hasAuthorship W1970202326A5014026964 @default.
• W1970202326 hasAuthorship W1970202326A5018520418 @default.
• W1970202326 hasAuthorship W1970202326A5050958375 @default.
• W1970202326 hasAuthorship W1970202326A5059953254 @default.
• W1970202326 hasAuthorship W1970202326A5074782661 @default.
• W1970202326 hasAuthorship W1970202326A5075919447 @default.
• W1970202326 hasConcept C104317684 @default.
• W1970202326 hasConcept C121332964 @default.
• W1970202326 hasConcept C12554922 @default.
• W1970202326 hasConcept C142613039 @default.
• W1970202326 hasConcept C150903083 @default.
• W1970202326 hasConcept C160756335 @default.
• W1970202326 hasConcept C185592680 @default.
• W1970202326 hasConcept C207001950 @default.
• W1970202326 hasConcept C2777477808 @default.
• W1970202326 hasConcept C2778067846 @default.
• W1970202326 hasConcept C55493867 @default.
• W1970202326 hasConcept C62520636 @default.
• W1970202326 hasConcept C86803240 @default.
• W1970202326 hasConcept C91881484 @default.
• W1970202326 hasConcept C96305047 @default.
• W1970202326 hasConceptScore W1970202326C104317684 @default.
• W1970202326 hasConceptScore W1970202326C121332964 @default.
• W1970202326 hasConceptScore W1970202326C12554922 @default.
• W1970202326 hasConceptScore W1970202326C142613039 @default.

• next