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- W1970231568 abstract "To develop a sensitive assay for screening compounds against hepatitis C virus (HCV).The proteolytic cleavage of NS3/4A on enhanced yellow fluorescent protein (eYFP)-mitochondrial antiviral signaling protein (MAVS) was examined by reporter enzyme secreted placental alkaline phosphatase (SEAP), which enabled us to perform ongoing monitoring of anti-HCV drugs through repeated chemiluminescence. Subcellular localization of eYFP-MAVS was assessed by fluorescence microscopy. Cellular localization and protein levels were examined by Western blotting.HCV NS3/4A protease cleaved eYFP-MAVS from mitochondria to block the activation of interferon (IFN)-β promoter, thus resulting in downregulation of SEAP activity. The decrease in SEAP activity was proportional to the dose of active NS3/4A protease. Also this reporter assay was used to detect anti-HCV activity of IFN-α and cyclosporine A.Our data show that this reporter system is a sensitive and quantitative reporter of anti-HCV inhibitors. This system will constitute a new tool to allow the efficient screening of HCV inhibitors." @default.
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- W1970231568 date "2010-01-01" @default.
- W1970231568 modified "2023-09-23" @default.
- W1970231568 title "Screening compounds against HCV based on MAVS/IFN-β pathway in a replicon model" @default.
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- W1970231568 doi "https://doi.org/10.3748/wjg.v16.i44.5582" @default.
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