FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1970258376> ?p ?o ?g. }

• W1970258376 endingPage "3397" @default.
• W1970258376 startingPage "3386" @default.
• W1970258376 abstract "Abstract We report a study on neural metabolism of long‐term three‐dimensional cultures of rat embryonic brain cells in stirred vessels. Our experimental setup was optimized to keep viable aggregate cultures with neuronal maintenance for up to 44 days. Results show that aggregate size and shape could be hydrodynamically controlled depending on the impeller design, avoiding necrotic centers or significant losses in cell viability. Aggregates were composed mainly of neurons until day 16, whereas an effective growth of the glial population was observed after day 21. Cell metabolic status was evaluated by quantification of several metabolites in the culture medium; amino acid metabolism was used as a marker of metabolic interrelationships between neural cell types. Furthermore, 13 C‐NMR spectroscopy was used on day 31 to explore specific metabolic pathways: incubation with [1‐ 13 C]glucose for 45 hr produced an increase in label incorporation in extracellular alanine, lactate, and glutamine, reflecting mainly astrocytic metabolism. The contribution of anaplerotic vs. oxidative pathways for glutamine synthesis was determined: a 92% reduction in the pyruvate carboxylase flux during the first 41 hr of incubation suggested a decrease in the need for replacing tricarboxylic acid cycle intermediates. We believe that our data corroborate the aggregating cultures as an attractive system to analyze brain cell metabolism being a valuable tool to model metabolic fluxes for in vitro brain diseases. © 2007 Wiley‐Liss, Inc." @default.
• W1970258376 created "2016-06-24" @default.
• W1970258376 creator A5021060711 @default.
• W1970258376 creator A5023299411 @default.
• W1970258376 creator A5041231390 @default.
• W1970258376 creator A5055789216 @default.
• W1970258376 creator A5074787856 @default.
• W1970258376 date "2007-07-12" @default.
• W1970258376 modified "2023-09-25" @default.
• W1970258376 title "Stirred vessel cultures of rat brain cells aggregates: Characterization of major metabolic pathways and cell population dynamics" @default.
• W1970258376 cites W1483940173 @default.
• W1970258376 cites W1569754812 @default.
• W1970258376 cites W1581874507 @default.
• W1970258376 cites W1908801241 @default.
• W1970258376 cites W1930183105 @default.
• W1970258376 cites W1968370763 @default.
• W1970258376 cites W1968419639 @default.
• W1970258376 cites W1970217675 @default.
• W1970258376 cites W1970742151 @default.
• W1970258376 cites W1971540171 @default.
• W1970258376 cites W1975399822 @default.
• W1970258376 cites W1986446573 @default.
• W1970258376 cites W1986660175 @default.
• W1970258376 cites W1987080089 @default.
• W1970258376 cites W1989249456 @default.
• W1970258376 cites W1990430727 @default.
• W1970258376 cites W1991648436 @default.
• W1970258376 cites W1996622604 @default.
• W1970258376 cites W2002802452 @default.
• W1970258376 cites W2018068740 @default.
• W1970258376 cites W2021742972 @default.
• W1970258376 cites W2024717575 @default.
• W1970258376 cites W2026796857 @default.
• W1970258376 cites W2026889931 @default.
• W1970258376 cites W2034615358 @default.
• W1970258376 cites W2045235878 @default.
• W1970258376 cites W2045367907 @default.
• W1970258376 cites W2050823048 @default.
• W1970258376 cites W2051823174 @default.
• W1970258376 cites W2055852420 @default.
• W1970258376 cites W2056409947 @default.
• W1970258376 cites W2058482175 @default.
• W1970258376 cites W2059729222 @default.
• W1970258376 cites W2069446568 @default.
• W1970258376 cites W2072304064 @default.
• W1970258376 cites W2078642866 @default.
• W1970258376 cites W2086914035 @default.
• W1970258376 cites W2108576625 @default.
• W1970258376 cites W2149672704 @default.
• W1970258376 cites W2160499404 @default.
• W1970258376 cites W2160906243 @default.
• W1970258376 cites W2164626711 @default.
• W1970258376 cites W2167596663 @default.
• W1970258376 cites W2217719122 @default.
• W1970258376 cites W2223085371 @default.
• W1970258376 cites W2326199983 @default.
• W1970258376 cites W962922507 @default.
• W1970258376 doi "https://doi.org/10.1002/jnr.21409" @default.
• W1970258376 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17628504" @default.
• W1970258376 hasPublicationYear "2007" @default.
• W1970258376 type Work @default.
• W1970258376 sameAs 1970258376 @default.
• W1970258376 citedByCount "18" @default.
• W1970258376 countsByYear W19702583762012 @default.
• W1970258376 countsByYear W19702583762016 @default.
• W1970258376 countsByYear W19702583762017 @default.
• W1970258376 countsByYear W19702583762018 @default.
• W1970258376 countsByYear W19702583762021 @default.
• W1970258376 countsByYear W19702583762022 @default.
• W1970258376 crossrefType "journal-article" @default.
• W1970258376 hasAuthorship W1970258376A5021060711 @default.
• W1970258376 hasAuthorship W1970258376A5023299411 @default.
• W1970258376 hasAuthorship W1970258376A5041231390 @default.
• W1970258376 hasAuthorship W1970258376A5055789216 @default.
• W1970258376 hasAuthorship W1970258376A5074787856 @default.
• W1970258376 hasConcept C144024400 @default.
• W1970258376 hasConcept C1491633281 @default.
• W1970258376 hasConcept C149923435 @default.
• W1970258376 hasConcept C160941953 @default.
• W1970258376 hasConcept C165135838 @default.
• W1970258376 hasConcept C192989942 @default.
• W1970258376 hasConcept C2779349466 @default.
• W1970258376 hasConcept C28406088 @default.
• W1970258376 hasConcept C2908647359 @default.
• W1970258376 hasConcept C515207424 @default.
• W1970258376 hasConcept C53227056 @default.
• W1970258376 hasConcept C54355233 @default.
• W1970258376 hasConcept C55493867 @default.
• W1970258376 hasConcept C62231903 @default.
• W1970258376 hasConcept C81885089 @default.
• W1970258376 hasConcept C86803240 @default.
• W1970258376 hasConcept C95444343 @default.
• W1970258376 hasConceptScore W1970258376C144024400 @default.
• W1970258376 hasConceptScore W1970258376C1491633281 @default.
• W1970258376 hasConceptScore W1970258376C149923435 @default.
• W1970258376 hasConceptScore W1970258376C160941953 @default.
• W1970258376 hasConceptScore W1970258376C165135838 @default.
• W1970258376 hasConceptScore W1970258376C192989942 @default.

• next