FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1970307788> ?p ?o ?g. }

• W1970307788 endingPage "548" @default.
• W1970307788 startingPage "543" @default.
• W1970307788 abstract "When small ligands are immobilized onto a porous chromatography medium, only a limited number of binding sites contributes to the interaction with the target molecule. The main part of the ligand molecules is distributed on sites that are not accessible for the target protein due to steric hindrance. To direct the ligand into a well-accessible position, the ligand was conjugated to a large molecule that acted as a placeholder during the immobilization step. Then the placeholder molecule was cleaved off and washed out. Two linear peptides with affinity for lysozyme and human blood coagulation factor VIII, respectively, were studied as model systems. The protected peptide ligand was covalently linked to a 20-kDa poly(ethylene glycol) molecule containing an acid-labile linker. After selective deprotection of the peptide and purification, immobilization of this conjugate on a preactivated chromatography matrix was performed alternatively through the free N-terminus, the epsilon-amino group of lysine, or the sulfohydryl group of cysteine. After the immobilization reaction, the spacer molecule and remaining protecting groups were cleaved off and the gels were tested by affinity chromatography. This novel immobilization technique substantially increased the binding capacity and the ligand utilization for the target protein, and site-specific immobilization could be demonstrated." @default.
• W1970307788 created "2016-06-24" @default.
• W1970307788 creator A5026284894 @default.
• W1970307788 creator A5036985567 @default.
• W1970307788 creator A5061734652 @default.
• W1970307788 creator A5068237857 @default.
• W1970307788 date "2002-12-21" @default.
• W1970307788 modified "2023-10-13" @default.
• W1970307788 title "Directed Immobilization of Peptide Ligands to Accessible Pore Sites by Conjugation with a Placeholder Molecule" @default.
• W1970307788 cites W185109643 @default.
• W1970307788 cites W1969427704 @default.
• W1970307788 cites W1975883810 @default.
• W1970307788 cites W1999839466 @default.
• W1970307788 cites W2022257442 @default.
• W1970307788 cites W2030689588 @default.
• W1970307788 cites W2041719513 @default.
• W1970307788 cites W2045292574 @default.
• W1970307788 cites W2087316284 @default.
• W1970307788 cites W2109509601 @default.
• W1970307788 cites W2148352345 @default.
• W1970307788 cites W2149464124 @default.
• W1970307788 cites W2213646474 @default.
• W1970307788 cites W4233003262 @default.
• W1970307788 doi "https://doi.org/10.1021/ac025846v" @default.
• W1970307788 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12585482" @default.
• W1970307788 hasPublicationYear "2002" @default.
• W1970307788 type Work @default.
• W1970307788 sameAs 1970307788 @default.
• W1970307788 citedByCount "35" @default.
• W1970307788 countsByYear W19703077882012 @default.
• W1970307788 countsByYear W19703077882013 @default.
• W1970307788 countsByYear W19703077882014 @default.
• W1970307788 countsByYear W19703077882016 @default.
• W1970307788 countsByYear W19703077882020 @default.
• W1970307788 countsByYear W19703077882023 @default.
• W1970307788 crossrefType "journal-article" @default.
• W1970307788 hasAuthorship W1970307788A5026284894 @default.
• W1970307788 hasAuthorship W1970307788A5036985567 @default.
• W1970307788 hasAuthorship W1970307788A5061734652 @default.
• W1970307788 hasAuthorship W1970307788A5068237857 @default.
• W1970307788 hasConcept C111919701 @default.
• W1970307788 hasConcept C112613896 @default.
• W1970307788 hasConcept C116569031 @default.
• W1970307788 hasConcept C134306372 @default.
• W1970307788 hasConcept C161624437 @default.
• W1970307788 hasConcept C170493617 @default.
• W1970307788 hasConcept C178790620 @default.
• W1970307788 hasConcept C179247698 @default.
• W1970307788 hasConcept C180577832 @default.
• W1970307788 hasConcept C181199279 @default.
• W1970307788 hasConcept C185592680 @default.
• W1970307788 hasConcept C197336794 @default.
• W1970307788 hasConcept C201194858 @default.
• W1970307788 hasConcept C21951064 @default.
• W1970307788 hasConcept C2777516009 @default.
• W1970307788 hasConcept C2779201268 @default.
• W1970307788 hasConcept C2779281246 @default.
• W1970307788 hasConcept C2780557392 @default.
• W1970307788 hasConcept C32909587 @default.
• W1970307788 hasConcept C33923547 @default.
• W1970307788 hasConcept C41008148 @default.
• W1970307788 hasConcept C521977710 @default.
• W1970307788 hasConcept C55493867 @default.
• W1970307788 hasConcept C71240020 @default.
• W1970307788 hasConceptScore W1970307788C111919701 @default.
• W1970307788 hasConceptScore W1970307788C112613896 @default.
• W1970307788 hasConceptScore W1970307788C116569031 @default.
• W1970307788 hasConceptScore W1970307788C134306372 @default.
• W1970307788 hasConceptScore W1970307788C161624437 @default.
• W1970307788 hasConceptScore W1970307788C170493617 @default.
• W1970307788 hasConceptScore W1970307788C178790620 @default.
• W1970307788 hasConceptScore W1970307788C179247698 @default.
• W1970307788 hasConceptScore W1970307788C180577832 @default.
• W1970307788 hasConceptScore W1970307788C181199279 @default.
• W1970307788 hasConceptScore W1970307788C185592680 @default.
• W1970307788 hasConceptScore W1970307788C197336794 @default.
• W1970307788 hasConceptScore W1970307788C201194858 @default.
• W1970307788 hasConceptScore W1970307788C21951064 @default.
• W1970307788 hasConceptScore W1970307788C2777516009 @default.
• W1970307788 hasConceptScore W1970307788C2779201268 @default.
• W1970307788 hasConceptScore W1970307788C2779281246 @default.
• W1970307788 hasConceptScore W1970307788C2780557392 @default.
• W1970307788 hasConceptScore W1970307788C32909587 @default.
• W1970307788 hasConceptScore W1970307788C33923547 @default.
• W1970307788 hasConceptScore W1970307788C41008148 @default.
• W1970307788 hasConceptScore W1970307788C521977710 @default.
• W1970307788 hasConceptScore W1970307788C55493867 @default.
• W1970307788 hasConceptScore W1970307788C71240020 @default.
• W1970307788 hasIssue "3" @default.
• W1970307788 hasLocation W19703077881 @default.
• W1970307788 hasLocation W19703077882 @default.
• W1970307788 hasOpenAccess W1970307788 @default.
• W1970307788 hasPrimaryLocation W19703077881 @default.
• W1970307788 hasRelatedWork W2012259422 @default.
• W1970307788 hasRelatedWork W2027377285 @default.
• W1970307788 hasRelatedWork W2048490996 @default.
• W1970307788 hasRelatedWork W2065110017 @default.
• W1970307788 hasRelatedWork W2073620476 @default.

• next