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- W1970324838 abstract "Sheep corpus luteum homogenates were fractionated by centrifugation on continuous sucrose density gradients, with or without digitonin, and gradient fractions were assayed for progesterone, and for a range of intracellular organelle and plasma-membrane markers. Digitonin had little effect on the density distributions of mitochondrial, rough endoplasmic reticulum (RER) and Golgi-endoplasmic reticulum-lysosomal (GERL) membranes. However, digitonin did disrupt lysosomal membranes, leading to release of acid hydrolases, and induced a decrease in buoyant density of NADH-cytochrome c reductase, a putative smooth endoplasmic reticulum (SER) marker. Oxytocin-containing granules were clearly resolved from other organelles accumulating in a sharp peak (density, 1.20 g/cm3). Luteal cell-surface membrane marker activities equilibrated at similar buoyant densities in control gradients, and pretreatment with digitonin induced a marked increase in their buoyant densities. The majority of the progesterone of the sheep corpus luteum equilibrated at a buoyant density of 1.10 g/cm3 in control gradients, and was highly perturbed by digitonin. These fractions also accumulated [3H]progesterone. The buoyant density profile of progesterone in both control and digitonin-treated gradients most closely resembled that of sheep luteal lactogenic receptor, a putative plasma-membrane marker." @default.
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- W1970324838 date "1988-09-01" @default.
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- W1970324838 title "Subcellular fractionation of the ovine corpus luteum: association of progesterone with ovine luteal membranes?" @default.
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- W1970324838 doi "https://doi.org/10.1016/0303-7207(88)90204-3" @default.
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