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• W1970331041 endingPage "e88792" @default.
• W1970331041 startingPage "e88792" @default.
• W1970331041 abstract "Transport of genetic materials and proteins between the nucleus and cytoplasm of eukaryotic cells is mediated by nuclear pore complexes (NPCs). A selective barrier formed by phenylalanine-glycine (FG) nucleoporins (Nups) with net positive charges in the NPC allows for passive diffusion of signal-independent small molecules and transport-receptor facilitated translocation of signal-dependent cargo molecules. Recently, negative surface charge was postulated to be another essential criterion for selective passage through the NPC. However, the charge-driven mechanism in determining the transport kinetics and spatial transport route for either passive diffusion or facilitated translocation remains obscure. Here we employed high-speed single-molecule fluorescence microscopy with an unprecedented spatiotemporal resolution of 9 nm and 400 µs to uncover these mechanistic fundamentals for nuclear transport of charged substrates through native NPCs. We found that electrostatic interaction between negative surface charges on transiting molecules and the positively charged FG Nups, although enhancing their probability of binding to the NPC, never plays a dominant role in determining their nuclear transport mode or spatial transport route. A 3D reconstruction of transport routes revealed that small signal-dependent endogenous cargo protein constructs with high positive surface charges that are destined to the nucleus, rather than repelled from the NPC as suggested in previous models, passively diffused through an axial central channel of the NPC in the absence of transport receptors. Finally, we postulated a comprehensive map of interactions between transiting molecules and FG Nups during nucleocytoplasmic transport by combining the effects of molecular size, signal and surface charge." @default.
• W1970331041 created "2016-06-24" @default.
• W1970331041 creator A5012369659 @default.
• W1970331041 creator A5023657528 @default.
• W1970331041 date "2014-02-18" @default.
• W1970331041 modified "2023-09-27" @default.
• W1970331041 title "Role of Molecular Charge in Nucleocytoplasmic Transport" @default.
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• W1970331041 doi "https://doi.org/10.1371/journal.pone.0088792" @default.
• W1970331041 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3928296" @default.
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• W1970331041 hasPublicationYear "2014" @default.
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