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• W1970352012 abstract "Background: The purpose of this study is to determine the frequency of the presence of B. pertussis among adolescent/adults who have prolonged cough for 2 weeks or longer. Methods & Materials: Two hundred and fourteen patients aged 10-39 years with the complaint of cough persisting for 2 weeks or longer were enrolled in the study. Nasofarengeal swab specimens were obtained from patients in the Departments of Pediatrics and Pulmonary Medicine at the Akdeniz University Hospital and in the Department of Pulmonary Medicine at the Antalya Education and Research Hospital between October 2010 and May 2011. Culture and PCR methods were performed to detect B. pertussis. IS481 real time PCR, and ptxA-Pr gene PCR were used for PCR analysis of B. pertussis. For the diagnosis of pertussis, IS481 real-time PCR and ptxA-Pr PCR were used in combination and the interpretation criteria of PCR results were as follows: if a specimen was positive for IS481 and ptxA-Pr it was considered to contain B. pertussis, if a specimen was positive for IS481 with a CT below 35 and negative for ptxA-Pr it was considered to contain Bordetella spp, if a specimen was positive for IS481 with a CT between 35 and 40 and negative for ptxA-Pr, it was considered indeterminate. Results: Three patients were B. pertussis culture-positive. A total of 51 samples (23%) were positive B. pertussis PCR-positive by IS481 real-time PCR. Fifteen samples were positive for B. pertussis by PCR method targeting ptxA-Pr gene. Using the interpretation criteria of PCR results; 15 specimens were interpreted as positive for B. pertussis, 11 specimens were interpreted as positive for Bordetella spp., 25 specimens were interpreted as indeterminate and 163 specimens were considered as negative. Conclusion: We conclude that B. pertussis infection is a common cause of persistant cough in adolescents and adults. PCR has several advantages over culture such as increased sensitivity and decreased time of the results. However, there are potential problems related to specificity and cross reactions that can complicate the interpretation of test results for pertussis diagnosis. Culture and PCR continue to have prominent places in the diagnostic process, but both have limitations." @default.
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• W1970352012 date "2014-04-01" @default.
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• W1970352012 title "Investigation of bordetella pertusis among patients aged 10-39 years with the complaint of cough persisting for two weeks or longer by culture and PCR" @default.
• W1970352012 doi "https://doi.org/10.1016/j.ijid.2014.03.931" @default.
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